Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,680	C→T	D310N (GAC→AAC)	pyrC ←	dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,680	0	C	T	100.0%	177.3 / NA	51	D310N (GAC→AAC)	pyrC	dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

CAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGC  >  NC_000913/1121545‑1121811                                                                                                                                        |                                                                                                                                    caGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTgttgtt                                                                                                                                  >  1:105637/1‑139 (MQ=255)    gAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGAc                                                                                                                               <  1:57371/139‑1 (MQ=255)       gCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCgg                                                                                                                            <  2:510063/139‑1 (MQ=255)         tACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCa                                                                                                                          <  1:565674/139‑1 (MQ=255)             aTTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAAccc                                                                                                                      >  1:294681/1‑139 (MQ=255)                tttATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATa                                                                                                                   >  1:346252/1‑139 (MQ=255)                  tATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAAc                                                                                                                        >  1:708192/1‑132 (MQ=255)                  tATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAAc                                                                                                                        <  2:708192/132‑1 (MQ=255)                          aaCGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCggg                                                                                                         <  2:294681/139‑1 (MQ=255)                          aaCGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCggg                                                                                                         <  2:346252/139‑1 (MQ=255)                              gACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGt                                                                                                     <  1:679209/139‑1 (MQ=255)                                 cAGCGTACCGTTTCCCCGGTGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                       <  1:414174/134‑1 (MQ=255)                                 cAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCa                                                                                                                          >  1:86362/1‑115 (MQ=255)                                 cAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCa                                                                                                                          <  2:86362/115‑1 (MQ=255)                                 cAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTa                                                                                                  >  2:679758/1‑139 (MQ=255)                                 cAGCGTACCGTGTCCCCGGTGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                       >  2:414174/1‑134 (MQ=255)                                         cGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaacag                                                                                          >  2:571284/1‑139 (MQ=255)                                          gTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaacaga                                                                                         <  2:360091/139‑1 (MQ=255)                                              ccccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCggg                                                                                                         <  1:370597/119‑1 (MQ=255)                                              ccccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCggg                                                                                                         >  2:370597/1‑119 (MQ=255)                                              ccccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTAcagaacagaa                                                                                        >  2:307196/1‑136 (MQ=255)                                              ccccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTAcagaacagaa                                                                                        <  1:307196/136‑1 (MQ=255)                                                              cACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCaaa                                                                     >  1:549125/1‑139 (MQ=255)                                                                   gCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCgg                                                                                                                            >  2:423158/1‑79 (MQ=255)                                                                   gCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCgg                                                                                                                            <  1:423158/79‑1 (MQ=255)                                                                    cGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGcattc                                                               >  1:310538/1‑139 (MQ=255)                                                                             agtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCa                                                      >  1:46526/1‑139 (MQ=255)                                                                               tcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCaaa                                                    <  1:571284/139‑1 (MQ=255)                                                                                   tGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCaaa                                                                               >  1:329526/1‑108 (MQ=255)                                                                                   tGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCaaa                                                                               <  2:329526/108‑1 (MQ=255)                                                                                   tGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACg                                                >  2:258667/1‑139 (MQ=255)                                                                                   tGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACg                                                >  2:704698/1‑139 (MQ=255)                                                                                       aTGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAg                                            <  2:105637/139‑1 (MQ=255)                                                                                       aTGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAg                                            <  1:258667/139‑1 (MQ=255)                                                                                         gCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCg                                          >  2:573000/1‑139 (MQ=255)                                                                                               aGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTg                                    <  2:46526/139‑1 (MQ=255)                                                                                                 cAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGa                                                  >  1:70017/1‑123 (MQ=255)                                                                                                 cAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGATGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGa                                                  <  2:70017/123‑1 (MQ=255)                                                                                                    ccTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGAc                                                 <  2:321373/121‑1 (MQ=255)                                                                                                    ccTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGAc                                                 >  1:321373/1‑121 (MQ=255)                                                                                                                  cGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGt                 >  1:461172/1‑139 (MQ=255)                                                                                                                     aCCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaacaga                                                                                         <  2:322204/64‑1 (MQ=255)                                                                                                                     aCCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaacaga                                                                                         >  1:322204/1‑64 (MQ=255)                                                                                                                        aGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAagc           >  1:505618/1‑139 (MQ=255)                                                                                                                          ttCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCg                  >  1:679276/1‑130 (MQ=255)                                                                                                                          ttCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCg                  <  2:679276/130‑1 (MQ=255)                                                                                                                          ttCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAagcag         >  2:312784/1‑139 (MQ=255)                                                                                                                                 aaTGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGAc                                                 <  1:494864/92‑1 (MQ=255)                                                                                                                                 aaTGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGAc                                                 >  2:494864/1‑92 (MQ=255)                                                                                                                                 aaTGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCgcgc  <  2:549125/139‑1 (MQ=255)                                                                                                                                   tgtgTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCcgc    >  2:202076/1‑135 (MQ=255)                                                                                                                                   tgtgTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCcgc    <  1:202076/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGC  >  NC_000913/1121545‑1121811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑