Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,520,008 C→T L328L (CTG→TTG)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,0080CT100.0% 40.5 / NA 13L328L (CTG→TTG) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

TGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCA  >  NC_000913/1519890‑1520137
                                                                                                                      |                                                                                                                                 
tGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGa                                                                                                                             >  1:104195/1‑125 (MQ=255)
tGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGa                                                                                                                             <  2:104195/125‑1 (MQ=255)
    cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  1:509578/139‑1 (MQ=255)
                                       aTGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  1:600569/104‑1 (MQ=255)
                                       aTGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           >  2:600569/1‑104 (MQ=255)
                                                    gATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGt                                                                                    >  1:269999/1‑114 (MQ=255)
                                                    gATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGt                                                                                    <  2:269999/114‑1 (MQ=255)
                                                                     cgAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAAt                                          >  1:300639/1‑139 (MQ=255)
                                                                                   aCGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCt                            >  2:48139/1‑139 (MQ=255)
                                                                                              aaTGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCt                 <  1:201471/139‑1 (MQ=255)
                                                                                              aaTGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCt                 <  1:602456/139‑1 (MQ=255)
                                                                                               aTGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCtt                >  2:470459/1‑139 (MQ=255)
                                                                                                             tttATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACca  <  1:470459/139‑1 (MQ=255)
                                                                                                                      |                                                                                                                                 
TGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCA  >  NC_000913/1519890‑1520137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: