Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,564,585	A→G	G383G (GGT→GGC)	dosP ←	oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated, cold‑ and stationary phase‑induced bioflim regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,564,585	0	A	G	100.0%	130.1 / NA	35	G383G (GGT→GGC)	dosP	oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated, cold‑ and stationary phase‑induced bioflim regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

CTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGACCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGC  >  NC_000913/1564450‑1564710                                                                                                                                        |                                                                                                                              cTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCg                                                                                                                            <  1:56819/139‑1 (MQ=255)      cGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCAt                                                                                                                       <  1:350056/139‑1 (MQ=255)           cACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGAt                                                                                                                  <  1:702182/139‑1 (MQ=255)                   gCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTgg                                                                                                          >  1:592279/1‑139 (MQ=255)                         gCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAgt                                                                                                    >  1:191228/1‑139 (MQ=255)                         gCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAgt                                                                                                    <  2:530120/139‑1 (MQ=255)                           tATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAgttg                                                                                                  <  2:494536/139‑1 (MQ=255)                                   acaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGAt                                                                                                                  <  2:247424/115‑1 (MQ=255)                                   acaTCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGAt                                                                                                                  >  1:247424/1‑115 (MQ=255)                                                 gTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATg                                                                                                       <  2:591704/112‑1 (MQ=255)                                                 gTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATg                                                                                                       >  1:591704/1‑112 (MQ=255)                                                         cGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTc                                                                    >  2:565696/1‑139 (MQ=255)                                                           aTGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCaa                                                                                                               >  1:66924/1‑94 (MQ=255)                                                           aTGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCaa                                                                                                               <  2:66924/94‑1 (MQ=255)                                                                      cacGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGAt                                                                                                        <  1:263215/90‑1 (MQ=255)                                                                      cacGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGAt                                                                                                        >  2:263215/1‑90 (MQ=255)                                                                                aCGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATgct                                             >  1:308046/1‑139 (MQ=255)                                                                                              cAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGcc                               <  2:592279/139‑1 (MQ=255)                                                                                               aGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCa                              <  1:199923/139‑1 (MQ=255)                                                                                                 gTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAgttgtt                                                                                                >  1:657225/1‑71 (MQ=255)                                                                                                 gTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAgttgtt                                                                                                <  2:657225/71‑1 (MQ=255)                                                                                                 gTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGAc                                                                                    >  2:388297/1‑83 (MQ=255)                                                                                                 gTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGAc                                                                                    <  1:388297/83‑1 (MQ=255)                                                                                                                gtgCAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCACGCGTTCGATAAAGgcg             <  1:565696/139‑1 (MQ=255)                                                                                                                   cAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCat                                                  <  1:707930/99‑1 (MQ=255)                                                                                                                   cAGGTTATTGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCat                                                  >  2:707930/1‑99 (MQ=255)                                                                                                                           tGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTccagcgccagcg                                                         <  1:679561/84‑1 (MQ=255)                                                                                                                           tGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTccagcgccagcg                                                         >  2:679561/1‑84 (MQ=255)                                                                                                                           tGCGATTTGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGc  <  1:231335/139‑1 (MQ=255)                                                                                                                                tttGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTcc                                                                         <  2:445727/63‑1 (MQ=255)                                                                                                                                tttGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTcc                                                                         >  1:445727/1‑63 (MQ=255)                                                                                                                                tttGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCACGCGTTCGATAAAGgcg             <  1:711086/123‑1 (MQ=255)                                                                                                                                tttGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCACGCGTTCGATAAAGgcg             >  2:711086/1‑123 (MQ=255)                                                                                                                                 ttGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCatat                                                >  2:550786/1‑87 (MQ=255)                                                                                                                                 ttGGCAGGCCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCatat                                                <  1:550786/87‑1 (MQ=255)                                                                                                                                        |                                                                                                                              CTGATCGGCCCACGCATAGCCAAGGCTATCAATCACATCCTGAATATGGTCAACACCGATGAGATACACCACGGGAGAGACGGCTTTGTCGACCAGGTCATCGAGGTAATTGTGCAGGTTATTGCGATTTGGCAGACCGGTCATCGGATCAAATTGGATGAGTTGTTCAATATGCTGACGGCTTTTTTCCTGTTCCAGCGCCAGCGCGGCCATATGCTGGCTGATATCTGCCACGCGTTCGATAAAGGCGCTGGTTTCTGC  >  NC_000913/1564450‑1564710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑