Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,359	T→C	I221T (ATC→ACC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,359	0	T	C	100.0%	126.2 / NA	34	I221T (ATC→ACC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/20);  total (14/20)

ATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGA  >  NC_000913/1756232‑1756484                                                                                                                                |                                                                                                                              aTCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAgg                                                                                                                    <  1:51732/139‑1 (MQ=255)     ttGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAgg                                                                                                                >  2:358259/1‑139 (MQ=255)            cGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTcaac                                                                                                         >  1:322300/1‑139 (MQ=255)              aaCAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTcaacaa                                                                                                       >  1:636705/1‑139 (MQ=255)                                      ctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCctc                                                                               >  1:104568/1‑139 (MQ=255)                                      ctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCctc                                                                               <  2:636705/139‑1 (MQ=255)                                         ccTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGa                                                                            <  1:358259/139‑1 (MQ=255)                                                     aGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGt                                                                >  1:123971/1‑139 (MQ=255)                                                         ttCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcg                                                            >  2:715852/1‑139 (MQ=255)                                                             gACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTg                                                        <  2:104568/139‑1 (MQ=255)                                                                 ttATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGa                                                    >  1:493080/1‑139 (MQ=255)                                                                  tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGATGCGCGTGGCGAc                                                   <  2:388335/139‑1 (MQ=255)                                                                   aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGAcc                                                  <  2:123971/139‑1 (MQ=255)                                                                   aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGATTGCGCGTGGCGAcc                                                  >  1:65213/1‑139 (MQ=255)                                                                    tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         <  1:114915/115‑1 (MQ=255)                                                                    tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         >  2:114915/1‑115 (MQ=255)                                                                    tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCt                                                 <  1:125485/139‑1 (MQ=255)                                                                    tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCt                                                 <  2:493080/139‑1 (MQ=255)                                                                       aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTggg                                              <  1:715852/139‑1 (MQ=255)                                                                       aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTggg                                              <  1:30196/139‑1 (MQ=255)                                                                                 gCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGATTGCGCGTGGCGACCTGGGTGTAGAAATc                                    <  2:65213/139‑1 (MQ=255)                                                                                      tGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGt                               >  1:536040/1‑139 (MQ=255)                                                                                                     gcgAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCg                                                                                      >  1:422786/1‑69 (MQ=255)                                                                                                     gcgAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCg                                                                                      <  2:422786/69‑1 (MQ=255)                                                                                                         aaaCATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTTTCTTCGcc            <  1:464805/139‑1 (MQ=255)                                                                                                            cATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCag         <  2:251319/139‑1 (MQ=255)                                                                                                            cATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCag         <  2:536040/139‑1 (MQ=255)                                                                                                                   atcatcTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGTGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga  <  2:322300/139‑1 (MQ=255)                                                                                                                       tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGAc                                                                           <  1:616990/62‑1 (MQ=255)                                                                                                                       tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGAc                                                                           >  2:616990/1‑62 (MQ=255)                                                                                                                       tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         >  2:700041/1‑64 (MQ=255)                                                                                                                       tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         <  1:700041/64‑1 (MQ=255)                                                                                                                        ctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                     >  1:651227/1‑99 (MQ=255)                                                                                                                        ctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                     <  2:651227/99‑1 (MQ=255)                                                                                                                                |                                                                                                                              ATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGA  >  NC_000913/1756232‑1756484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑