| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,087,919 | C→T | A114T (GCT→ACT) | yeeY ← | LysR family putative transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,087,919 | 0 | C | T | 82.5% | 83.0 / 14.4 | 40 | A114T (GCT→ACT) | yeeY | LysR family putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (4/3); new base T (18/15); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
GATGGTGGCTCATGCATCGCGCCAACAATAATATCCGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCC > NC_000913/2087795‑2088051 | gATGGTGGCTCATGCATCGCGCCAACAATAATATCCGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGgcg > 2:617634/1‑139 (MQ=255)gATGGTGGCTCATGCATCGCGCCAACAATAATATCCGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGgcg < 2:325750/139‑1 (MQ=255) gggtCATGCATCGCGCCAACAATAATATCCGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCa < 2:475204/136‑1 (MQ=255) tGCATCGCGCCAACAATAATATCCGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGaaa > 2:238055/1‑139 (MQ=255) ccGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGcc > 1:87712/1‑139 (MQ=255) gCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCaa > 2:156064/1‑139 (MQ=255) tCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACgtg > 2:356539/1‑124 (MQ=255) tCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACgtg < 1:356539/124‑1 (MQ=255) gggCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGt > 1:289221/1‑83 (MQ=255) gggCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGt < 2:289221/83‑1 (MQ=255) gCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTccc < 1:156064/139‑1 (MQ=255) tCCCATGAACCACCGAGTACGCCATTGATAAACTTGAGGCGCGTCACGCTCTGATGTTGATAAAGAGTCTCAATGAGTGGAGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGc > 2:502460/1‑139 (MQ=255) cccATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGc > 1:20948/1‑86 (MQ=255) cccATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGc < 2:20948/86‑1 (MQ=255) tGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCa < 1:617634/139‑1 (MQ=255) accGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGt > 2:97323/1‑139 (MQ=255) ccGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCATGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGtt > 1:131893/1‑139 (MQ=255) aGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGgcg > 2:323362/1‑67 (MQ=255) aGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGgcg < 1:323362/67‑1 (MQ=255) tACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACgtg < 2:623047/88‑1 (MQ=255) tACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACgtg > 1:623047/1‑88 (MQ=255) cATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATg < 2:603999/124‑1 (MQ=255) cATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATg > 1:603999/1‑124 (MQ=255) aTAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCACGCAGTTTGATCGCCTGCtttt > 2:567978/1‑139 (MQ=255) ggCGCGTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCg > 2:56739/1‑139 (MQ=255) cgcgTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCATGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCa < 2:131893/139‑1 (MQ=255) cgcgTCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCa < 1:201103/139‑1 (MQ=255) cgTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACg < 1:238055/139‑1 (MQ=255) tCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGa < 2:70992/56‑1 (MQ=255) tCACGCTATGATGTTGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGa > 1:70992/1‑56 (MQ=255) ttGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTcc < 1:567978/139‑1 (MQ=255) tGATAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTccc < 2:351504/139‑1 (MQ=255) aTAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTccc > 1:671113/1‑77 (MQ=255) aTAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTccc < 2:671113/77‑1 (MQ=255) aTAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTc < 1:659931/108‑1 (MQ=255) aTAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTc > 2:659931/1‑108 (MQ=255) aTAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGc > 2:393777/1‑139 (MQ=255) aTAAAAAGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGc > 2:585215/1‑139 (MQ=255) aTAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGc > 2:677010/1‑139 (MQ=255) aaaaaGTTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGccc > 1:147575/1‑139 (MQ=255) | GATGGTGGCTCATGCATCGCGCCAACAATAATATCCGCTCGCCCCTGAGTCAGGGCATCCCATGAACCACCGAGTACGCCATTGATAAACTTCAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCC > NC_000913/2087795‑2088051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |