Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,842,591 T→C L151L (TTA→TTG hycI ← protease involved in processing C‑terminal end of HycE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,842,5910TC100.0% 100.0 / NA 27L151L (TTA→TTGhycIprotease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/14);  total (13/14)

ACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTT  >  NC_000913/2842464‑2842724
                                                                                                                               |                                                                                                                                     
aCGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGaa                                                                                                                            <  2:463748/139‑1 (MQ=255)
                       cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCAttt                                                                                                                  <  2:674440/126‑1 (MQ=255)
                       cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCAttt                                                                                                                  >  1:674440/1‑126 (MQ=255)
                       cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttccttcc                                                                                                            >  1:344069/1‑132 (MQ=255)
                       cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttccttcc                                                                                                            <  2:344069/132‑1 (MQ=255)
                                                  ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                                          >  2:374040/1‑139 (MQ=255)
                                                  ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                                          <  1:374040/139‑1 (MQ=255)
                                                                         tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATc                                                   >  1:381874/1‑139 (MQ=255)
                                                                           aaGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCgg                                                 <  1:416089/139‑1 (MQ=255)
                                                                                   cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                       <  2:670771/93‑1 (MQ=255)
                                                                                   cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                       >  1:670771/1‑93 (MQ=255)
                                                                                   cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                                          >  1:693786/1‑106 (MQ=255)
                                                                                   cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                                          <  2:693786/106‑1 (MQ=255)
                                                                                       gTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTaa                                                                 <  1:56098/111‑1 (MQ=255)
                                                                                       gTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTaa                                                                 >  2:56098/1‑111 (MQ=255)
                                                                                         ccTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCcga                                   <  2:279641/139‑1 (MQ=255)
                                                                                           tGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTa                                                                  <  1:462291/106‑1 (MQ=255)
                                                                                           tGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTa                                                                  >  2:462291/1‑106 (MQ=255)
                                                                                             aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttccttcc                                                                                                            >  1:206512/1‑62 (MQ=255)
                                                                                             aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttccttcc                                                                                                            <  2:206512/62‑1 (MQ=255)
                                                                                             aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat                               >  2:491531/1‑139 (MQ=255)
                                                                                                       gAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGa                     <  1:491531/139‑1 (MQ=255)
                                                                                                                      ttcCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCg                                                  >  2:315145/1‑95 (MQ=255)
                                                                                                                      ttcCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCg                                                  <  1:315145/95‑1 (MQ=255)
                                                                                                                          aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                                          >  2:714371/1‑67 (MQ=255)
                                                                                                                          aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCg                                                                          <  1:714371/67‑1 (MQ=255)
                                                                                                                          aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACtt  >  2:170622/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                     
ACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTT  >  NC_000913/2842464‑2842724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: