| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,911,083 | G→A | S3S (AGC→AGT) | mazF ← | mRNA interferase toxin, antitoxin is MazE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,911,083 | 0 | G | A | 82.1% | 120.4 / 22.6 | 56 | S3S (AGC→AGT) | mazF | mRNA interferase toxin, antitoxin is MazE |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base A (23/23); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
ACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAAT > NC_000913/2910946‑2911217 | acacacAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc < 2:456137/139‑1 (MQ=255) acacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACtt > 1:630510/1‑139 (MQ=255) acacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACtt > 1:422468/1‑139 (MQ=255) tACCTGTTTTGTTGTTTTACATGAAAGGACTCAGGAAAACAGCTGGACGATGTCAAGCTTGCTCGTTACCTTTGGTCGGGTAAAAATCAACCCAAATAAGATCGCCCATTTCGGGTACGTATCGACttaccattacaga < 1:292977/139‑4 (MQ=255) tACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAg > 1:188046/1‑139 (MQ=255) tACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAg < 2:188046/139‑1 (MQ=255) aCCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGa > 2:642554/1‑139 (MQ=255) gTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTc > 2:378385/1‑139 (MQ=255) tttGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt > 1:577333/1‑139 (MQ=255) ttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCttt < 1:161848/139‑1 (MQ=255) tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt > 2:711617/1‑131 (MQ=255) tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt < 1:711617/131‑1 (MQ=255) tGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGctct > 2:107023/1‑139 (MQ=255) gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCtctc > 1:517234/1‑139 (MQ=255) aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcc < 1:305140/139‑1 (MQ=255) tCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGGCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCg < 2:630510/139‑1 (MQ=255) aGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGat > 1:330989/1‑139 (MQ=255) aGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGat > 2:650270/1‑139 (MQ=255) caGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCg > 1:298249/1‑139 (MQ=255) ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc > 1:89773/1‑119 (MQ=255) ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAg > 2:119588/1‑139 (MQ=255) ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACACAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc < 2:89773/119‑1 (MQ=255) tGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc < 2:586213/113‑1 (MQ=255) tGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc > 1:586213/1‑113 (MQ=255) ttGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGa < 1:107023/139‑1 (MQ=255) tCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTc > 1:307756/1‑139 (MQ=255) tCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTc > 2:14658/1‑139 (MQ=255) cGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCg < 2:557403/139‑1 (MQ=255) cTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGtt < 2:422468/139‑1 (MQ=255) aCCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGa < 1:650270/139‑1 (MQ=255) aCCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGa < 2:330989/139‑1 (MQ=255) tGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc < 1:313232/53‑1 (MQ=255) tGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc > 2:313232/1‑53 (MQ=255) gggTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGc < 2:517234/139‑1 (MQ=255) ggTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGc > 1:423841/1‑115 (MQ=255) ggTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGc < 2:423841/115‑1 (MQ=255) gTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCaa > 1:439876/1‑139 (MQ=255) gTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCaa < 1:642554/139‑1 (MQ=255) aaaTCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc < 1:378385/139‑1 (MQ=255) aaaTCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc < 2:33817/139‑1 (MQ=255) aTCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCCCTCCCCAGTCGATATTCTCGTGGAGGGTTTCCGGCGTGATGTCGTTGACCAGTTCAGCATGCGTAACTACGGGTTCTTTAc > 2:354187/1‑139 (MQ=255) aTCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTAc > 2:655937/1‑139 (MQ=255) gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcccc < 1:337179/61‑1 (MQ=255) gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcccc > 2:337179/1‑61 (MQ=255) gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCa < 2:307756/139‑1 (MQ=255) gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCa < 2:298249/139‑1 (MQ=255) ccATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACggg < 2:479380/126‑1 (MQ=255) ccATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACggg > 1:479380/1‑126 (MQ=255) cGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGt < 2:611379/50‑1 (MQ=255) cGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGt > 1:611379/1‑50 (MQ=255) aTCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAAtaattaat > 1:641996/1‑139 (MQ=255) aTCGACTTACCATTACCAGACTTCGTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACgg > 2:484124/1‑110 (MQ=255) aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACgg < 1:484124/110‑1 (MQ=255) aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc < 2:164704/112‑1 (MQ=255) aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc > 1:164704/1‑112 (MQ=255) aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAAtaattaat > 2:92105/1‑139 (MQ=255) | ACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAAT > NC_000913/2910946‑2911217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |