Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 265,137 C→T V24I (GTC→ATC)  yafX ← CP4‑6 prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,1370CT100.0% 116.9 / NA 35V24I (GTC→ATC) yafXCP4‑6 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

AGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGACCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATAAAGTGTG  >  NC_000913/265002‑265269
                                                                                                                                       |                                                                                                                                    
aGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCgg                                                                                                                                   >  2:416324/1‑139 (MQ=255)
aGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCgg                                                                                                                                   >  1:520951/1‑139 (MQ=255)
                 ccGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCaa                                                                                                                  >  2:313489/1‑139 (MQ=255)
                              cacaGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCgg                                                                                                            <  1:167544/132‑1 (MQ=255)
                              cacaGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCgg                                                                                                            >  2:167544/1‑132 (MQ=255)
                                 aGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGc                                                                                                  <  1:27362/139‑1 (MQ=255)
                                 aGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGc                                                                                                  <  2:677827/139‑1 (MQ=255)
                                        gTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCg                                                                                           <  1:437782/139‑1 (MQ=255)
                                        gTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCg                                                                                           >  1:489271/1‑139 (MQ=255)
                                                             tggCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGtgtt                                                                      <  2:503060/138‑1 (MQ=255)
                                                                 tCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCa                                                                  <  1:79133/139‑1 (MQ=255)
                                                                   cAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCt                                                                           <  1:577549/128‑1 (MQ=255)
                                                                   cAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCt                                                                           >  2:577549/1‑128 (MQ=255)
                                                                            ccACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGtct                                                       >  1:174155/1‑139 (MQ=255)
                                                                                          cgcCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTc                                                                            <  1:672680/104‑1 (MQ=255)
                                                                                          cgcCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTc                                                                            >  2:672680/1‑104 (MQ=255)
                                                                                              aaaGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCa                                     <  2:489271/139‑1 (MQ=255)
                                                                                                       tgGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGAtg                            >  2:705710/1‑139 (MQ=255)
                                                                                                         gCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAgcggcg                               <  1:318465/134‑1 (MQ=255)
                                                                                                         gCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAgcggcg                               >  2:318465/1‑134 (MQ=255)
                                                                                                          ccAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCtgtg                                                                        >  1:136810/1‑92 (MQ=255)
                                                                                                          ccAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCtgtg                                                                        <  2:136810/92‑1 (MQ=255)
                                                                                                            aGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTGCGTCAATAAAAACGCCAGCGGCGAtggctgg                       >  2:688167/1‑139 (MQ=255)
                                                                                                                aTCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTa                   <  2:104521/139‑1 (MQ=255)
                                                                                                                aTCGATAAGCTGTTCATCGGGGATCGGCCTGACGGTAAGTTCAAATTCGGGTTGGTTAGCGGGGGCGGGGTCGTGTTGCTTCTGGGTTGTCAGTGGTATGTCCCAGTCAATAAAAACGCCAGCGGCGATTTCTCGCGTa                   <  2:492591/139‑1 (MQ=21)
                                                                                                                  cGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCTATTTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGGCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCTATGGCTGGCGTATg                 <  2:520951/139‑1 (MQ=21)
                                                                                                                    aTACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATggg               <  2:174155/139‑1 (MQ=255)
                                                                                                                           gTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAgcggcg                               >  1:711816/1‑116 (MQ=255)
                                                                                                                           gTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAgcggcg                               <  2:711816/116‑1 (MQ=255)
                                                                                                                            ttCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATaaa       <  1:416324/139‑1 (MQ=255)
                                                                                                                              cATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATAAAgt     <  1:705710/139‑1 (MQ=255)
                                                                                                                               aTCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATg                 <  2:620369/126‑1 (MQ=255)
                                                                                                                               aTCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATg                 >  1:620369/1‑126 (MQ=255)
                                                                                                                                 cGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATAAAgtgtg  >  2:537611/1‑139 (MQ=255)
                                                                                                                                   gggATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCtgtg                                                                        >  2:110571/1‑67 (MQ=255)
                                                                                                                                   gggATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCtgtg                                                                        <  1:110571/67‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
AGAACGACCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGACCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATATAAAGTGTG  >  NC_000913/265002‑265269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑