| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,123,022 | C→T | E262K (GAA→AAA) | glcB ← | malate synthase G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,123,022 | 0 | C | T | 84.6% | 27.9 / ‑2.2 | 13 | E262K (GAA→AAA) | glcB | malate synthase G |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (9/2); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCG > NC_000913/3122890‑3123155 | cttttttCTCCATTTTCATTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCCACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGAt < 1:494057/137‑1 (MQ=255) tctTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGTCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTgcg > 1:36071/1‑139 (MQ=255) agcagGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCAt > 1:460669/1‑139 (MQ=255) gTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGTCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCa < 2:36071/139‑1 (MQ=255) cagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGAt < 1:598967/78‑1 (MQ=255) cagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGAt > 2:598967/1‑78 (MQ=255) gATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCa > 1:140707/1‑139 (MQ=255) cGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCg > 2:466275/1‑139 (MQ=255) cATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGtt > 1:411698/1‑139 (MQ=255) gcgACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCa > 1:25843/1‑139 (MQ=255) gCAGTCGAGAATGGTACTGATAGCAGCTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGgc > 1:424414/1‑139 (MQ=255) aaTGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCa > 2:625075/1‑139 (MQ=255) agcTTTGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCg > 1:338443/1‑139 (MQ=255) | CGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCG > NC_000913/3122890‑3123155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |