Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,530,091	G→A	T106T (ACG→ACA)	hslO →	heat shock protein Hsp33

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,530,091	0	G	A	100.0%	23.2 / NA	9	T106T (ACG→ACA)	hslO	heat shock protein Hsp33
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

ACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGC  >  NC_000913/3530021‑3530224                                                                       |                                                                                                                                      aCAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGt                                                                   >  2:657303/1‑139 (MQ=255)   aaTAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCACGAGCGAAGGCGAACGCTATCAGGGCGTAGTTg                                                                 >  1:633076/1‑139 (MQ=255)                           gcgcgcGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGAAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTggcgg                                         <  2:633076/139‑1 (MQ=255)                                 gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                                             <  1:38245/65‑1 (MQ=255)                                 gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                                             >  2:38245/1‑65 (MQ=255)                                   gCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                                             <  1:638076/63‑1 (MQ=255)                                   gCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                                             >  2:638076/1‑63 (MQ=255)                                   gCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGcctgcctg                                 <  1:657303/139‑1 (MQ=255)                                                                  aaaaCACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGc  >  2:664417/1‑139 (MQ=255)                                                                       |                                                                                                                                      ACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGC  >  NC_000913/3530021‑3530224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑