Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,098	G→T	intergenic (‑23/+155)	yjcF ← / ← actP	pentapeptide repeats protein/acetate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,098	0	G	T	100.0%	60.1 / NA	19	intergenic (‑23/+155)	yjcF/actP	pentapeptide repeats protein/acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/9);  total (10/9)

CTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAG  >  NC_000913/4282966‑4283231                                                                                                                                     |                                                                                                                                      cTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAataaagaa                                                                                                                                 <  1:194757/139‑1 (MQ=255)     aaTTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAgg                                                                                                                             >  2:569116/1‑139 (MQ=255)           cTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTACAATTAACaataaagactgtctcttat                                                                                                                       >  2:454752/1‑128 (MQ=255)            tGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATtc                                                                                                                      <  1:680518/139‑1 (MQ=255)              aTTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGACATATtctc                                                                                                                    >  2:138241/1‑139 (MQ=255)                  tGTTTTCTTTGTAGGTGATAGACTTTTGACAGAGTGGTTTTCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa                                                                                                                <  2:310314/139‑1 (MQ=255)                        cTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCa                                                                                                          >  1:473697/1‑139 (MQ=255)                                   aTGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGAc                                                                                               >  2:76839/1‑139 (MQ=255)                                   aTGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGAc                                                                                               >  1:475292/1‑139 (MQ=255)                                             gACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAAt                                                                                     >  1:225317/1‑139 (MQ=255)                                                         aTCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCAtttttt                                                                         >  1:258123/1‑139 (MQ=255)                                                                      cAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTAttt                                                            >  1:27098/1‑139 (MQ=255)                                                                                  aaCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAAtattat                                                <  1:76839/139‑1 (MQ=255)                                                                                       attattTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGaa                                           <  1:567266/139‑1 (MQ=255)                                                                                             tAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTg                                     <  2:225317/139‑1 (MQ=255)                                                                                                           gcATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTc                                                                                <  1:353378/82‑1 (MQ=255)                                                                                                           gcATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTc                                                                                >  2:353378/1‑82 (MQ=255)                                                                                                            cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTa                                                                                                   <  2:186081/62‑1 (MQ=255)                                                                                                            cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTa                                                                                                   >  1:186081/1‑62 (MQ=255)                                                                                                               aaaaTATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGaa                   <  2:27098/139‑1 (MQ=255)                                                                                                                                aaCAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAg  <  2:41369/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      CTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAG  >  NC_000913/4282966‑4283231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑