| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,401,304 | T→C | S211S (TCT→TCC) | hflX → | GTPase, stimulated by 50S subunit binding, Mn(2+) homeostasis regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,401,304 | 0 | T | C | 88.2% | 100.0 / 2.0 | 34 | S211S (TCT→TCC) | hflX | GTPase, stimulated by 50S subunit binding, Mn(2+) homeostasis regulator |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (14/16); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TAATCGCATCGTGCAGATACAGTCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCTACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTT > NC_000913/4401175‑4401435 | tAATCGCATCGTGCAGATACAGTCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTc < 2:652976/139‑1 (MQ=255) aTCGTGCAGATACAGTCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCa < 2:351632/139‑1 (MQ=255) gTCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCgg < 2:596710/139‑1 (MQ=255) tCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAATTCCACCCTTTTCAATCg > 1:457371/1‑122 (MQ=255) tCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAATTCCACCCTTTTCAATCg < 2:457371/122‑1 (MQ=255) tCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCTACCCTTTTCAATCGCATCACCGAAgc < 2:598221/134‑1 (MQ=255) tCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCTACCCTTTTCAATCGCATCACCGAAgc > 1:598221/1‑134 (MQ=255) cgcgCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGt < 2:262018/139‑1 (MQ=255) gcCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTa > 1:102795/1‑139 (MQ=255) agTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGAc > 1:58368/1‑139 (MQ=255) gTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGAcc < 1:469919/139‑1 (MQ=255) aaaaGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAgtt > 2:126733/1‑139 (MQ=255) gggCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCgg > 2:566915/1‑112 (MQ=255) gggCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCgg < 1:566915/112‑1 (MQ=255) gcAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCTACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCgg > 2:536977/1‑107 (MQ=255) gcAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCTACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCgg < 1:536977/107‑1 (MQ=255) aaGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGa < 2:58368/139‑1 (MQ=255) cGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGtt < 1:126733/139‑1 (MQ=255) cGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCa > 1:416190/1‑139 (MQ=255) tACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTgcg < 2:552944/117‑1 (MQ=255) tACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTgcg > 1:552944/1‑117 (MQ=255) tCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGAt < 2:5167/129‑1 (MQ=255) tCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGAt > 1:5167/1‑129 (MQ=255) tCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGaaa < 2:416190/139‑1 (MQ=255) tCGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGaaa > 2:529842/1‑139 (MQ=255) cGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCa < 1:686741/48‑1 (MQ=255) cGCTGGTGGGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCa > 2:686741/1‑48 (MQ=255) ggtggGATATACCAACGCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTa < 1:529842/139‑1 (MQ=255) gCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAgc < 1:597417/38‑1 (MQ=255) gCCGGTAAATCCACCCTTTTCAATCGCATCACCGAAgc > 2:597417/1‑38 (MQ=255) ggTAAATCCACCCTTTTCAATCGCATCACCGAAgcg > 1:64406/1‑36 (MQ=255) ggTAAATCCACCCTTTTCAATCGCATCACCGAAgcg < 2:64406/36‑1 (MQ=255) gTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGtt > 1:557149/1‑139 (MQ=255) gTAAATCCACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGtt > 2:601194/1‑139 (MQ=255) | TAATCGCATCGTGCAGATACAGTCGCGCCTGGAAAGAGTTGAAAAGCAGCGTGAGCAGGGGCGGCAATCGCGTATCAAAGCCGACGTTCCTACTGTTTCGCTGGTGGGATATACCAACGCCGGTAAATCTACCCTTTTCAATCGCATCACCGAAGCGCGGGTCTACGCGGCAGACCAGTTGTTTGCCACCCTCGACCCGACGTTGCGGCGTATTGACGTTGCAGATGTCGGTGAAACCGTACTTGCAGATACCGTAGGGTT > NC_000913/4401175‑4401435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |