Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	749,989	A→G	G727G (GGT→GGC)	ybgQ ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	749,989	0	A	G	100.0%	113.0 / NA	30	G727G (GGT→GGC)	ybgQ	putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

GCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGG  >  NC_000913/749860‑750080                                                                                                                                  |                                                                                            gCCAACCTTACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATcgcg                                                                                    <  2:542277/139‑1 (MQ=255)     aCCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCtt                                                                                <  1:183484/139‑1 (MQ=255)      ccATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTc                                                                               >  1:14410/1‑139 (MQ=255)                gCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGtt                                                                     >  2:52719/1‑139 (MQ=255)                gCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGtt                                                                     >  2:509083/1‑139 (MQ=255)                gCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGtt                                                                     >  1:473191/1‑139 (MQ=255)                 cTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGttt                                                                    >  2:252461/1‑139 (MQ=255)                    aTGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCt                                                                 >  2:176927/1‑139 (MQ=255)                                    ggCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGc                                                 <  2:473191/139‑1 (MQ=255)                                       ggATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAAc                                              >  2:118393/1‑139 (MQ=255)                                           atCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGtt                                          <  1:52719/139‑1 (MQ=255)                                                 aCCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCg                                    <  2:506953/139‑1 (MQ=255)                                                     aggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCgg                                <  1:509083/139‑1 (MQ=255)                                                     aggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCgg                                >  2:227345/1‑139 (MQ=255)                                                      ggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCggg                               <  2:14410/139‑1 (MQ=255)                                                           gggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGt                          <  2:552801/139‑1 (MQ=255)                                                             gaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtca                        >  1:87565/1‑139 (MQ=255)                                                                   aCCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCaa                                                                             <  2:98384/80‑1 (MQ=255)                                                                   aCCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCaa                                                                             >  1:98384/1‑80 (MQ=255)                                                                   aCCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATg                  >  2:285/1‑139 (MQ=255)                                                                     cGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGtt                >  1:2637/1‑139 (MQ=255)                                                                        tGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCAc             >  2:332804/1‑139 (MQ=255)                                                                           cGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGc          <  1:285/139‑1 (MQ=255)                                                                            gTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGcc         <  1:227345/139‑1 (MQ=255)                                                                             ttGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCa        <  2:2637/139‑1 (MQ=255)                                                                                cGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGt                          >  2:297381/1‑118 (MQ=255)                                                                                cGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGt                          <  1:297381/118‑1 (MQ=255)                                                                                   atgatgACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTgg  <  1:118393/139‑1 (MQ=255)                                                                                               aCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGtt                                          >  2:535140/1‑87 (MQ=255)                                                                                               aCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGtt                                          <  1:535140/87‑1 (MQ=255)                                                                                                                                  |                                                                                            GCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGG  >  NC_000913/749860‑750080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑