Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	989,708	T→C	T217A (ACA→GCA)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,708	0	T	C	100.0%	83.0 / NA	23	T217A (ACA→GCA)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

ATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAA  >  NC_000913/989572‑989819                                                                                                                                         |                                                                                                                aTAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTc                                                                                                               >  1:373428/1‑139 (MQ=255)      gATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATc                                                                                                          >  1:350769/1‑139 (MQ=255)        tGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCgg                                                                                                        >  1:614365/1‑139 (MQ=255)            gTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTg                                                                                                    >  1:180456/1‑139 (MQ=255)                        aatgcCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGa                                                                                        <  2:373428/139‑1 (MQ=255)                                  tGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGAt                                                                              <  1:229327/139‑1 (MQ=255)                                    gTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCAt                                                                                                           <  2:4978/108‑1 (MQ=255)                                    gTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCAt                                                                                                           >  1:4978/1‑108 (MQ=255)                                    gTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCg                                                                            >  1:280337/1‑139 (MQ=255)                                       gggATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAg                                                                         <  2:367556/139‑1 (MQ=255)                                        ggATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGt                                                                        <  2:614365/139‑1 (MQ=255)                                                          gcagcaAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAAcc                                                      >  1:573909/1‑139 (MQ=255)                                                                 gTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTc                                               <  2:180456/139‑1 (MQ=255)                                                                          gctggctgTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAg                                      <  2:49784/139‑1 (MQ=255)                                                                           ctggctgTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGa                                     <  2:303072/139‑1 (MQ=255)                                                                                         gATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGt                       <  2:280337/139‑1 (MQ=255)                                                                                               ggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATg                         <  1:508081/131‑1 (MQ=255)                                                                                               ggCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATg                         >  2:508081/1‑131 (MQ=255)                                                                                                              gtgCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGaaaa  >  1:302433/1‑139 (MQ=255)                                                                                                                   tGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGt                       <  2:174389/113‑1 (MQ=255)                                                                                                                   tGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGt                       >  1:174389/1‑113 (MQ=255)                                                                                                                           ttCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGc                                        >  2:16866/1‑88 (MQ=255)                                                                                                                           ttCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGc                                        <  1:16866/88‑1 (MQ=255)                                                                                                                                         |                                                                                                                ATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAA  >  NC_000913/989572‑989819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑