Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	989,319	(G)5→6	coding (1038/1203 nt)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,314	1	.	G	100.0%	194.0 / NA	50	Q348P (CAG→CCG)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (25/25);  total (25/25)

GGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCT‑GGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTACTCTGTGGAT  >  NC_000913/989176‑989446                                                                                                                                            |                                                                                                                                     ggAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCt                                                                                                                                       >  2:451371/1‑139 (MQ=255)        cGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCT‑gggggga                                                                                                                               <  1:451371/139‑3 (MQ=255)        cGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCT‑gggggga                                                                                                                               >  1:838905/1‑137 (MQ=255)             gCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCg                                                                                                                           <  2:394694/139‑1 (MQ=255)               tttGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCa                                                                                                                         >  1:502366/1‑139 (MQ=255)                 tGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAgg                                                                                                                       >  1:117339/1‑139 (MQ=255)                      aGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAgg                                                                                                                  <  1:566797/139‑1 (MQ=255)                        cgcCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCg                                                                                                                >  2:425578/1‑139 (MQ=255)                               aCAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTTCCa                                                                                                         <  2:754712/139‑1 (MQ=255)                                   aCGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCGACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATAc                                                                                                     <  1:34067/139‑1 (MQ=255)                                                        ttttGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCggg                                                                                <  2:192619/139‑1 (MQ=255)                                                           tGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAag                                                                             >  2:155442/1‑139 (MQ=255)                                                              cAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCg                                                                                                                           <  1:318380/90‑1 (MQ=255)                                                              cAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCg                                                                                                                           >  2:318380/1‑90 (MQ=255)                                                                  gCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTg                                                                      >  1:34939/1‑139 (MQ=255)                                                                  gCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTg                                                                      >  2:448813/1‑139 (MQ=255)                                                                   cTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                                                     <  1:448813/139‑1 (MQ=255)                                                                   cTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                                                     <  2:838905/139‑1 (MQ=255)                                                                         gAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtat                                                               <  2:1048111/139‑1 (MQ=255)                                                                                tCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCT‑gggggg                                                                                                                                <  1:98298/66‑2 (MQ=255)                                                                                tCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCT‑gggggg                                                                                                                                >  2:98298/1‑65 (MQ=255)                                                                                          tttACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCg                                              <  1:425578/139‑1 (MQ=255)                                                                                          tttACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCg                                              <  2:363877/139‑1 (MQ=255)                                                                                                gTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTGAATc                                        <  2:117339/139‑1 (MQ=255)                                                                                                   acacTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAg                                     >  1:358375/1‑139 (MQ=255)                                                                                                   acacTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAg                                     >  1:284991/1‑139 (MQ=255)                                                                                                         tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAagag                                                                           <  1:470579/95‑1 (MQ=255)                                                                                                         tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAagag                                                                           >  2:470579/1‑95 (MQ=255)                                                                                                         tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTc                               >  1:275130/1‑139 (MQ=255)                                                                                                           ccAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCag                             >  2:202446/1‑139 (MQ=255)                                                                                                            cAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga                            >  2:539669/1‑139 (MQ=255)                                                                                                                 tAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCa                                                                                      <  2:1049483/76‑1 (MQ=255)                                                                                                                 tAATGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCa                                                                                      >  1:1049483/1‑76 (MQ=255)                                                                                                                   aTGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                                                  >  1:372810/1‑110 (MQ=255)                                                                                                                   aTGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                                                  <  2:372810/110‑1 (MQ=255)                                                                                                                   aTGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATc                                        >  2:19697/1‑120 (MQ=255)                                                                                                                   aTGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATc                                        <  1:19697/120‑1 (MQ=255)                                                                                                                   aTGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAAcc                     >  2:627932/1‑139 (MQ=255)                                                                                                                    tGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCa                    <  2:275130/139‑1 (MQ=255)                                                                                                                    tGACAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCa                    <  1:539669/139‑1 (MQ=255)                                                                                                                       cAATATTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCg                 <  1:942375/139‑1 (MQ=255)                                                                                                                          tatTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa                                    <  1:1053821/117‑1 (MQ=255)                                                                                                                          tatTCAGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa                                    >  2:1053821/1‑117 (MQ=255)                                                                                                                               aGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTActc         >  2:511386/1‑139 (MQ=255)                                                                                                                               aGGGGTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTActc         >  2:271353/1‑139 (MQ=255)                                                                                                                                 gggTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTACTCtg       >  1:696304/1‑139 (MQ=255)                                                                                                                                 gggTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTACTCtg       >  2:703046/1‑139 (MQ=255)                                                                                                                                 gggTTTTACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTACTCtg       <  1:155442/139‑1 (MQ=255)                                                                                                                                    ttttACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTAct          <  1:481730/133‑1 (MQ=255)                                                                                                                                    ttttACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTAct          >  2:481730/1‑133 (MQ=255)                                                                                                                                      ttACCTGGGGGGATATCGCCGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGtt               >  1:801044/1‑126 (MQ=255)                                                                                                                                      ttACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCg                                              >  1:261716/1‑95 (MQ=255)                                                                                                                                      ttACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCg                                              <  2:261716/95‑1 (MQ=255)                                                                                                                                      ttACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGtt               <  2:801044/126‑1 (MQ=255)                                                                                                                                      ttACCTGGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTACTCTGTGGAt  <  2:358375/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     GGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGGGCTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCT‑GGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAGAAAACCAGCGTTTTACTCTGTGGAT  >  NC_000913/989176‑989446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑