Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,351,196 | (G)7→8 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,351,189 | 1 | . | G | 96.4% | 196.0 / 0.1 | 55 | intergenic (‑150/+218) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (27/26); total (28/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01 |
TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGT > NC_000913/1351052‑1351323 | tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑g > 2:483268/1‑139 (MQ=255) gATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTACAAAGCCGTATGAGTAGAAAAACGGGTATTACAATCAAGCTGGCGGTGAGTATCTATAGAAGCAGGGTCCGACCGGAGCAGGCCAGGGCA‑‑ggcggcgg < 1:61097/139‑4 (MQ=255) aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑ggggggggc > 1:1885/1‑137 (MQ=255) gCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑ggggggggcgc > 2:20448/1‑135 (MQ=255) ttgtTCCTGAAAATCAGGCGATTCGTCGTGTTAGTAAACAGTACGAACATATAAACGGTTATTATAATCAACCTGGCTGTGAGGAGCTATACTTGCCAGGTCTGACCGAATCAGGCTGCGGCAG‑GGGGGGGCGCTTTTc > 1:789650/1‑139 (MQ=255) gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 1:872244/139‑1 (MQ=255) gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCt < 2:528059/139‑1 (MQ=255) ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTc > 1:1088343/1‑139 (MQ=255) tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCg > 2:984601/1‑139 (MQ=255) aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa < 2:1088343/139‑1 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct > 1:190793/1‑114 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct < 2:190793/114‑1 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt > 2:1103500/1‑139 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 2:1885/139‑1 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgggg > 1:311837/1‑115 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgggg < 2:311837/115‑1 (MQ=255) aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc > 2:796077/1‑87 (MQ=255) aTCAACCTGGATGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc < 1:796077/87‑1 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa > 2:153860/1‑139 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGC‑TTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa > 1:483373/1‑139 (MQ=255) ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGATTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa > 1:168785/1‑139 (MQ=255) cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa < 1:153860/139‑1 (MQ=255) cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt > 1:164394/1‑109 (MQ=255) cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt < 2:164394/109‑1 (MQ=255) ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa < 2:894655/97‑1 (MQ=255) ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa > 1:894655/1‑97 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGTGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc < 1:1068162/78‑1 (MQ=255) gCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGTGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAAcc > 2:1068162/1‑78 (MQ=255) gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa > 1:1040904/1‑101 (MQ=255) gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 2:1040904/101‑1 (MQ=255) aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCAt > 2:236310/1‑139 (MQ=255) gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTcc > 2:459755/1‑75 (MQ=38) gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTcc < 1:459755/75‑1 (MQ=38) gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCTCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAg > 2:459068/1‑139 (MQ=255) gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga < 1:168532/79‑1 (MQ=255) gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga > 2:168532/1‑79 (MQ=255) taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa < 2:88826/82‑1 (MQ=255) taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa > 1:88826/1‑82 (MQ=255) gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa > 1:296245/1‑139 (MQ=255) tGCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAc > 2:914642/1‑139 (MQ=255) gCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa > 1:344379/1‑95 (MQ=255) gCCAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa < 2:344379/95‑1 (MQ=255) ccAGGTCCGACCGGAGGAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc < 1:100849/65‑1 (MQ=37) ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc > 2:100849/1‑65 (MQ=255) ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa > 2:676504/1‑76 (MQ=255) ccAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa < 1:676504/76‑1 (MQ=255) cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt > 1:392339/1‑139 (MQ=255) cAGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt < 1:459068/139‑1 (MQ=255) aGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct < 1:329141/58‑1 (MQ=38) aGGTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCct > 2:329141/1‑58 (MQ=38) gTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCACTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc < 1:1039043/123‑1 (MQ=255) gTCCGACCGGAGCAGGCTGCGGCAG‑GGGGGGGCACTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATAc > 2:1039043/1‑123 (MQ=255) ccGGAGCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg < 1:236310/139‑1 (MQ=255) gCAGGCTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGc < 2:392339/139‑1 (MQ=255) cTGCGGCAG‑GGGGGGGCTCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 1:20448/139‑1 (MQ=255) cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 2:296245/139‑1 (MQ=255) cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 1:984601/139‑1 (MQ=255) cTGCGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAAAATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa < 1:590683/139‑1 (MQ=255) cGGCAG‑GGGGGGGCGCTTTTCCCCTCACCCTAA‑CCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGt > 1:387672/1‑139 (MQ=255) | TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGT > NC_000913/1351052‑1351323 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |