Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,421,650 (C)7→8 coding (532/858 nt) ydaU → Rac prophage, conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,421,6431.C95.3% 156.5 / 0.2 43coding (525/858 nt)ydaURac prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (25/16);  total (26/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

ACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGAACGGAG  >  NC_000913/1421518‑1421775
                                                                                                                              |                                                                                                                                     
acaaaacaaGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCcggggg                                                                                                                          >  1:632846/1‑138 (MQ=255)
   aaacaaGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGAT‑‑CCCCCTAAATCCCCCCCCGGGGGAATCg                                                                                                                      <  2:422272/139‑1 (MQ=255)
    aacaaGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATc                                                                                                                       >  1:1062066/1‑139 (MQ=255)
                    aaCAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATccccccccgg                                                                                                                             >  1:15514/1‑113 (MQ=255)
                    aaCAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCcgg                                                                                                                             <  2:15514/116‑1 (MQ=255)
                      cAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTg                                                                                                     <  2:482978/139‑1 (MQ=255)
                        ggTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGAc                                                                                                   >  2:873686/1‑139 (MQ=255)
                           gtTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                <  2:335327/139‑1 (MQ=255)
                            ttCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCctc                                                                                               >  1:301785/1‑139 (MQ=255)
                                    gATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                                                                      <  1:52432/124‑1 (MQ=255)
                                    gATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGttt                                                                                                      >  2:52432/1‑124 (MQ=255)
                                      tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                >  1:26733/1‑128 (MQ=255)
                                      tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                <  2:26733/128‑1 (MQ=255)
                                      tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATcc                                                                                                                                     >  1:520380/1‑90 (MQ=255)
                                      tGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATcc                                                                                                                                     <  2:520380/90‑1 (MQ=255)
                                          gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTg                                                                                 <  2:632846/139‑1 (MQ=255)
                                          gAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTg                                                                                 >  1:562121/1‑139 (MQ=255)
                                                gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGgt                                                                                                                  <  2:837151/100‑1 (MQ=255)
                                                gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGgt                                                                                                                  >  1:837151/1‑100 (MQ=255)
                                                gtgtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAAc                                                                           >  2:947068/1‑139 (MQ=255)
                                                  gtTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTg                                                                         <  2:1062066/139‑1 (MQ=255)
                                                          atgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTg                                                                 >  2:495420/1‑139 (MQ=255)
                                                           tgatgTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGt                                                                >  2:181045/1‑139 (MQ=255)
                                                                       aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                <  1:542944/95‑1 (MQ=255)
                                                                       aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                >  2:542944/1‑95 (MQ=255)
                                                                       aaaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCg                                                    >  1:411457/1‑139 (MQ=255)
                                                                        aaGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGt                                                   >  2:120404/1‑139 (MQ=255)
                                                                         aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTg                                                  >  2:222159/1‑139 (MQ=255)
                                                                         aGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTg                                                  >  1:873034/1‑139 (MQ=255)
                                                                              aCTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTgg                                             >  1:101395/1‑139 (MQ=255)
                                                                                    aaaGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGaa                                       <  2:873034/139‑1 (MQ=255)
                                                                                      aGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAAtt                                     <  1:181045/139‑1 (MQ=255)
                                                                                          aCAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCaa                                                                                                             <  1:1009674/63‑1 (MQ=255)
                                                                                          aCAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCaa                                                                                                             >  2:1009674/1‑63 (MQ=255)
                                                                                            aGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                >  1:212976/1‑74 (MQ=255)
                                                                                            aGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCct                                                                                                <  2:212976/74‑1 (MQ=255)
                                                                                                      cTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGa                     >  2:568754/1‑139 (MQ=255)
                                                                                                      cTAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGa                     >  1:122957/1‑139 (MQ=255)
                                                                                                       tAAAAACAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGaa                    >  1:125795/1‑139 (MQ=255)
                                                                                                        aaaaaCAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt                                                          <  1:863066/100‑1 (MQ=255)
                                                                                                        aaaaaCAGATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCt                                                          >  2:863066/1‑100 (MQ=255)
                                                                                                               gATCCCCCCCTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGAtt           >  1:804527/1‑139 (MQ=255)
                                                                                                                   ccccccTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGaa       <  2:122957/139‑1 (MQ=255)
                                                                                                                       ccTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCCGATTCGAACgg    >  2:853371/1‑139 (MQ=255)
                                                                                                                        cTAAATCCCCCCCCGGGGGAATCGGGGGGGCAAAAAGGTTGGCCCTCTGGATATTACTTTTCCGAAATGGAATTCTGTCTCGCTTTTGCGGGAGGGGGGTTAATTTCGGCCGGCATTGCGAAAACCGATTCGAACgggg  >  2:764403/1‑137 (MQ=255)
                                                                                                                        cTAAATCCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGAACGGAg  <  1:947068/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
ACAAAACAAGAGAGAAATTTAACAGGTGTTCAAACAGATGTTGAAGTGGTGTTTGAACATGATGTCAACACAAAGGCAACTAATAAAGATACAGATAAAGATCTAAAAACAGATCCCCCCCTAAATCCCCCCCGGGGGAATCGAGGTGTCAAAAAGTTTGACCCTCTGGATATTACTTTGCCGAACTGGATTTCTGTCTCGCTTTGGCGTGAGTGGGTTGAATTTCGCCAGGCATTGCGAAAACCGATTCGAACGGAG  >  NC_000913/1421518‑1421775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: