| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,194,362 | (G)6→7 | coding (1499/1662 nt) | yqiK → | PHB family membrane protein, function unknown |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,194,356 | 1 | . | G | 93.8% | 106.0 / 0.5 | 32 | coding (1493/1662 nt) | yqiK | PHB family membrane protein, function unknown |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (15/15); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
CCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑GGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCC > NC_000913/3194224‑3194486 | ccTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑gggggg > 2:765555/1‑139 (MQ=255) cTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑ggggggg < 1:765555/139‑2 (MQ=255) cgcTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTgg < 2:647145/139‑1 (MQ=255) aaTCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCg < 1:830023/139‑1 (MQ=255) tCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCTTTATCAGCCGct < 2:10011/139‑1 (MQ=255) ttGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCgg > 2:17584/1‑115 (MQ=255) ttGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCgg < 1:17584/115‑1 (MQ=255) tGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGctctctc > 2:534951/1‑139 (MQ=255) aTGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGc > 2:901897/1‑139 (MQ=255) cAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAgg < 1:959782/139‑1 (MQ=255) aTCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGAtt < 2:548706/139‑1 (MQ=255) aTCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGAtt < 1:800341/139‑1 (MQ=255) attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTgg > 1:1040869/1‑77 (MQ=255) attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTgg < 2:1040869/77‑1 (MQ=255) attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGct > 1:83158/1‑100 (MQ=255) attCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGct < 2:83158/100‑1 (MQ=255) gTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc < 1:261878/116‑1 (MQ=255) gTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc > 2:261878/1‑116 (MQ=255) tGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAAt > 2:5964/1‑139 (MQ=255) gAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGt > 1:721370/1‑139 (MQ=255) tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCg > 1:452889/1‑75 (MQ=255) tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCg < 2:452889/75‑1 (MQ=255) tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg > 2:991690/1‑139 (MQ=255) tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg > 1:899177/1‑139 (MQ=255) tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg < 1:163913/139‑1 (MQ=255) tGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCCCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAgg > 1:681001/1‑139 (MQ=255) ggcggcGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc > 2:599542/1‑98 (MQ=255) ggcggcGCTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc < 1:599542/98‑1 (MQ=255) ggcggcGCTGCGGGTGATGCAAACACGGGTAATGT‑GGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc > 2:676464/1‑97 (MQ=255) ggcggcGCTGCGGGTGATGCAAACACGGGTAATGT‑GGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCAc < 1:676464/97‑1 (MQ=255) gcgcTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAAc > 2:480397/1‑55 (MQ=255) gcgcTGCGGGTGATGCAAACACGGGTAATGTGGGGGGGCGGAAACCTGGCGGAAc < 1:480397/55‑1 (MQ=255) acGGGTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCa < 2:138214/139‑1 (MQ=255) ggTAATGTGGGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCAccc > 2:1064747/1‑139 (MQ=255) | CCTTGTTGCAGGCGCTGCCTGCGGTAATAGAGAAATCCGTTGAGCCGATGAAGTCAATCGACGGTATCAAGATTATTCAGGTCGATGGTCTGAATCGTGGCGGCGCTGCGGGTGATGCAAACACGGGTAATGT‑GGGGGGCGGAAACCTGGCGGAACAAGCATTATCAGCCGCTCTCTCTTACCGCACACAGGCACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCC > NC_000913/3194224‑3194486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |