Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	593,467	T→G	D435A (GAC→GCC)	cusS ←	copper‑sensing histidine kinase in two‑component regulatory system with CusR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	593,467	0	T	G	100.0%	134.9 / NA	38	D435A (GAC→GCC)	cusS	copper‑sensing histidine kinase in two‑component regulatory system with CusR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/19);  total (19/19)

GCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTG  >  NC_000913/593332‑593596                                                                                                                                        |                                                                                                                                  gCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCaa                                                                                                                                <  2:319411/139‑1 (MQ=255)           tgATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGaa                                                                                                                      <  2:147642/139‑1 (MQ=255)                    aCCTTGTCCCCCGCGCATCCGACGTTATCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTAACGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGAGATAGAAACGGCCaaa                                                                                                             >  1:1097223/1‑139 (MQ=255)                    aCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCaaa                                                                                                             >  2:101988/1‑139 (MQ=255)                    aCCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCaaa                                                                                                             >  1:254154/1‑139 (MQ=255)                     ccTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAAc                                                                                                            >  1:740061/1‑139 (MQ=255)                          tCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCaa                                                                                                                                <  2:62573/114‑1 (MQ=255)                          tCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCaa                                                                                                                                >  1:62573/1‑114 (MQ=255)                          tCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATcg                                                                                                       >  2:681089/1‑139 (MQ=255)                               cgcgCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTa                                                                                                  >  2:22290/1‑139 (MQ=255)                                     tCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCt                                                                                            <  1:460708/139‑1 (MQ=255)                                     tCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCt                                                                                            <  2:254154/139‑1 (MQ=255)                                              aCCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCaa                                                                                   >  2:304601/1‑139 (MQ=255)                                                          ccTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGt                                                                                                   <  1:160548/111‑1 (MQ=255)                                                          ccTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGt                                                                                                   >  2:160548/1‑111 (MQ=255)                                                               aTGTGCGACAACAATCGATTGCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCaa                                                                                                                                >  2:753808/1‑77 (MQ=255)                                                               aTGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCaa                                                                                                                                <  1:753808/77‑1 (MQ=255)                                                                     gACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTcgacg                                                            <  2:1047636/139‑1 (MQ=255)                                                                      acaacaATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTcgacga                                                           <  2:636723/139‑1 (MQ=255)                                                                         acaaTCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATaa                                                        <  1:749719/139‑1 (MQ=255)                                                                                 tttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTcgacg                                                            >  1:189966/1‑127 (MQ=255)                                                                                 tttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTcgacg                                                            <  2:189966/127‑1 (MQ=255)                                                                                 tttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCAcc                                                <  2:529764/139‑1 (MQ=255)                                                                                 tttCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCAcc                                                >  1:529764/1‑139 (MQ=255)                                                                                       tATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGa                                          >  1:156201/1‑139 (MQ=255)                                                                                                gCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTc                                 >  2:28122/1‑139 (MQ=255)                                                                                                     tACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACa                            <  1:28122/139‑1 (MQ=255)                                                                                                          cTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAg                           >  2:766502/1‑135 (MQ=255)                                                                                                          cTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAg                           <  1:766502/135‑1 (MQ=255)                                                                                                          cTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCa                       >  1:335059/1‑139 (MQ=255)                                                                                                          cTACCTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCa                       >  2:456444/1‑139 (MQ=255)                                                                                                              cTTCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACTGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTAc                   <  2:1097223/139‑1 (MQ=255)                                                                                                               ttCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGTGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGt                                            <  2:893000/113‑1 (MQ=255)                                                                                                               ttCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGTGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGt                                            >  1:893000/1‑113 (MQ=255)                                                                                                                tCACCTTTTCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACaa                 <  1:101988/139‑1 (MQ=255)                                                                                                                       ttCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctc          >  1:775810/1‑139 (MQ=255)                                                                                                                        tCGCTGGCGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGtctct         <  1:681089/139‑1 (MQ=255)                                                                                                                               cGGGAGGGGGCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTg  <  2:977207/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  GCGGGTAATGTGATAACAAACCTTGTCCCCCGCGCATCCGACGTTACCGCAACCGTGCCTTTATGCGCGACAACAATCGATTTCACTATCGCCAGGCCAATACCGCTACCTTCACCTTTTCGCTGGCGGGAGGGGTCAACGCGATAGAAACGGTCAAACAATCGCGGTAAGTGCTCGGGCGCAATGGGCGTACCGGGGTTTTCGACGATAACTTGCACCAGGTGATCGACCGTCTGACAGCGCACTACAATTGTCTCTCCGGTTG  >  NC_000913/593332‑593596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑