Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 600,470 C→T A586V (GCG→GTG)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913600,4700CT100.0% 79.9 / NA 24A586V (GCG→GTG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/12);  total (12/12)

TTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACG  >  NC_000913/600348‑600591
                                                                                                                          |                                                                                                                         
ttCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTAACGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCTCAGCAGAGGCGGTGAGTATGCTGCaaaaa                                                                                                           >  2:750200/1‑139 (MQ=255)
     tGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGa                                                                                                      <  2:897019/139‑1 (MQ=255)
                 tctGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTAt                                                                                          <  1:1021605/139‑1 (MQ=255)
                    ggCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg                                                                                       <  2:235917/139‑1 (MQ=255)
                    ggCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCAACAAGCTAATTATGAg                                                                                       <  1:485173/139‑1 (MQ=255)
                       cGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGt                                                                                    >  1:398195/1‑139 (MQ=255)
                         cTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAg                                                                                                  >  1:278047/1‑123 (MQ=255)
                         cTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAg                                                                                                  <  2:278047/123‑1 (MQ=255)
                          tCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTAcc                                                                                 >  1:954381/1‑139 (MQ=255)
                               aaaGTTGGCGGGGAATTTTTACGTCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAg                                                                            <  1:291526/139‑1 (MQ=255)
                                                                         ttgttgTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCa                                  >  2:466450/1‑139 (MQ=255)
                                                                                 tatGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCa                                                                                                               >  1:721344/1‑54 (MQ=255)
                                                                                 tatGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCa                                                                                                               <  2:721344/54‑1 (MQ=255)
                                                                                    gCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGGGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCt                       >  2:735336/1‑139 (MQ=255)
                                                                                      cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGa                                                                                                      >  1:598161/1‑58 (MQ=255)
                                                                                      cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGa                                                                                                      <  2:598161/58‑1 (MQ=255)
                                                                                      cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCt                                                                                                <  2:528664/64‑1 (MQ=255)
                                                                                      cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCt                                                                                                >  1:528664/1‑64 (MQ=255)
                                                                                        tCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGc                   >  2:847571/1‑139 (MQ=255)
                                                                                         cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGt                                                                                                                       >  1:691427/1‑38 (MQ=255)
                                                                                         cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGt                                                                                                                       <  2:691427/38‑1 (MQ=255)
                                                                                         cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCt                  <  2:954381/139‑1 (MQ=255)
                                                                                            cGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGga               <  1:466450/139‑1 (MQ=255)
                                                                                                         ttCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACg  >  1:615881/1‑139 (MQ=255)
                                                                                                                      gcggtgAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAAcc                                      <  2:1010466/90‑1 (MQ=255)
                                                                                                                      gcggtgAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAAcc                                      >  1:1010466/1‑90 (MQ=255)
                                                                                                                          |                                                                                                                         
TTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACG  >  NC_000913/600348‑600591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: