Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	857,298	C→T	R446W (CGG→TGG)	ybiT →	ABC‑F family putative regulatory ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	857,298	0	C	T	100.0%	181.4 / NA	53	R446W (CGG→TGG)	ybiT	ABC‑F family putative regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/29);  total (24/29)

TGTTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAA  >  NC_000913/857164‑857408                                                                                                                                       |                                                                                                               tgtTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGG‑AAAAGGGTGGATg                                                                                                           <  2:890231/139‑1 (MQ=255)  gtTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATg                                                                                                           >  1:1045247/1‑139 (MQ=255)  gtTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATg                                                                                                           >  2:830225/1‑139 (MQ=255)   ttCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGc                                                                                                          <  2:302442/139‑1 (MQ=255)   ttCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGc                                                                                                          <  2:325850/139‑1 (MQ=255)       aaTGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGtt                                                                                                      >  2:942898/1‑139 (MQ=255)          ggATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                   <  1:1028213/139‑1 (MQ=255)          ggATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                   <  2:489955/139‑1 (MQ=255)           gATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGt                                                                                                  <  1:30201/139‑1 (MQ=255)           gATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACACAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGt                                                                                                  >  1:787098/1‑139 (MQ=255)            aTGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGATGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGTGTGGATGCTGTTTGGTa                                                                                                 >  2:683404/1‑139 (MQ=255)                 ccAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTa                                                                                            >  1:863512/1‑139 (MQ=255)                     tGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAatga                                                                                        >  2:1091133/1‑139 (MQ=255)                              gAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                   <  1:435418/119‑1 (MQ=255)                              gAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                   >  2:435418/1‑119 (MQ=255)                               aaGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGcc                                                                              >  2:42442/1‑139 (MQ=255)                                 ggCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGa                                                                            >  2:988094/1‑139 (MQ=255)                                 ggCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGa                                                                            <  1:942898/139‑1 (MQ=255)                                  gCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGaa                                                                           <  2:584177/139‑1 (MQ=255)                                      tGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACAtt                                                                       <  1:749465/139‑1 (MQ=255)                                           aGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGAt                                                                  >  1:809400/1‑139 (MQ=255)                                                      cGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGttt                                                                                                     <  2:545336/93‑1 (MQ=255)                                                      cGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGttt                                                                                                     >  1:545336/1‑93 (MQ=255)                                                          gTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGac                                                   <  1:988094/139‑1 (MQ=255)                                                           tATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGacc                                                  <  1:830225/139‑1 (MQ=255)                                                             ttCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGaccaa                                                <  2:821465/139‑1 (MQ=255)                                                                 cGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAaccac                                            <  1:1709/139‑1 (MQ=255)                                                                  ggTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAaccacc                                           <  1:89871/139‑1 (MQ=255)                                                                  ggTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAaccacc                                           <  2:1045247/139‑1 (MQ=255)                                                                   gTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCt                                          >  1:317178/1‑139 (MQ=255)                                                                   gTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCt                                          <  2:809400/139‑1 (MQ=255)                                                                   gTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCt                                          >  1:837188/1‑139 (MQ=255)                                                                     cGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAGTGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTgg                                        <  1:683404/139‑1 (MQ=255)                                                                     cGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTgg                                        >  1:185357/1‑139 (MQ=255)                                                                     cGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTgg                                        >  1:254396/1‑139 (MQ=255)                                                                       tttGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGat                                      >  1:529443/1‑139 (MQ=255)                                                                                  gCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCAt                           >  1:187464/1‑139 (MQ=255)                                                                                    cAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTTTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTg                         <  1:1091133/139‑1 (MQ=255)                                                                                        acgacATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                   >  2:517341/1‑61 (MQ=255)                                                                                        acgacATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                   <  1:517341/61‑1 (MQ=255)                                                                                        acgacATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAg                                                                                   >  2:127945/1‑77 (MQ=255)                                                                                        acgacATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAg                                                                                   <  1:127945/77‑1 (MQ=255)                                                                                            cATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACAtt                                                                       <  1:962221/85‑1 (MQ=255)                                                                                            cATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACAtt                                                                       >  2:962221/1‑85 (MQ=255)                                                                                                aaaaaGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACa             <  2:317178/139‑1 (MQ=255)                                                                                                aaaaaGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACa             <  2:837188/139‑1 (MQ=255)                                                                                                         gCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGAt                                                                  <  2:571682/77‑1 (MQ=255)                                                                                                         gCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGAt                                                                  >  1:571682/1‑77 (MQ=255)                                                                                                          cTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGa   >  1:472814/1‑139 (MQ=255)                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa  <  2:254396/139‑1 (MQ=255)                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa  <  2:185357/139‑1 (MQ=255)                                                                                                              aGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGaccaacca                                             >  2:17805/1‑93 (MQ=255)                                                                                                              aGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGaccaacca                                             <  1:17805/93‑1 (MQ=255)                                                                                                                                       |                                                                                                               TGTTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAA  >  NC_000913/857164‑857408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑