Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 936,202 C→T T993T (ACC→ACT)  ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913936,2020CT100.0% 108.0 / NA 34T993T (ACC→ACT) ftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

CGCGAAGTTTTGGATAACGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCAT  >  NC_000913/936068‑936334
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cgcgAAGTTTTGGATAACGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGtt                                                                                                                                  >  1:88757/1‑139 (MQ=255)
cgcgAAGTTTTGGATAACGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGtt                                                                                                                                  >  1:205658/1‑139 (MQ=255)
  cgAAGTTTTGGATAACGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTcc                                                                                                                                >  2:790996/1‑139 (MQ=255)
                 cGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGgt                                                                                                                 >  2:955525/1‑139 (MQ=255)
                          ccGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAAcgcg                                                                                                        <  2:88757/139‑1 (MQ=255)
                           cgcgATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGt                                                                                                                             <  1:327776/117‑1 (MQ=255)
                           cgcgATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGt                                                                                                                             >  2:327776/1‑117 (MQ=255)
                                      gTCGCCATTAACCGTGGTGGTGGGTAAAGATATCGCCGGGGATGCGGGGGTTGCCGAACTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGc                                                                                            <  1:255182/139‑1 (MQ=255)
                                      gTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGc                                                                                            <  2:685707/139‑1 (MQ=255)
                                         gCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATg                                                                                         <  1:955525/139‑1 (MQ=255)
                                               aaCCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTtat                                                                                   >  1:399357/1‑139 (MQ=255)
                                                    tggtgCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCga                                                                                                       >  1:35408/1‑114 (MQ=255)
                                                    tggtgCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCga                                                                                                       <  2:35408/114‑1 (MQ=255)
                                                     ggtgCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGca                                                                             >  1:392964/1‑139 (MQ=255)
                                                     ggtgCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGca                                                                             >  1:1111409/1‑139 (MQ=255)
                                                     ggtgCTAGGTAAAGATATTGCCGGTGAGACGGTGATTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGca                                                                             <  2:712164/139‑1 (MQ=255)
                                                                aaGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCagaaga                                                                  >  2:623749/1‑139 (MQ=255)
                                                                 aGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGAt                                                                 <  1:790996/139‑1 (MQ=255)
                                                                      tCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGt                                                                                                                     >  2:430533/1‑82 (MQ=255)
                                                                      tCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGt                                                                                                                     <  1:430533/82‑1 (MQ=255)
                                                                      tCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCg                                                            >  1:1013118/1‑139 (MQ=255)
                                                                       cGCCGGTTACCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGt                                                           <  1:201624/139‑1 (MQ=255)
                                                                                 ccGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGa                                                 >  2:873995/1‑139 (MQ=255)
                                                                                  cGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGAt                                                <  1:878849/139‑1 (MQ=255)
                                                                                          gCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGa                                        <  1:873995/139‑1 (MQ=255)
                                                                                                 tGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCt                                 <  2:205658/139‑1 (MQ=255)
                                                                                                         aTGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCttt                         <  2:614723/139‑1 (MQ=255)
                                                                                                                           gCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTc       <  2:671788/139‑1 (MQ=255)
                                                                                                                            cGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGAt                                                >  1:1005323/1‑97 (MQ=255)
                                                                                                                            cGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGAt                                                <  2:1005323/97‑1 (MQ=255)
                                                                                                                              gggACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGaa              >  2:871424/1‑129 (MQ=255)
                                                                                                                              gggACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGaa              <  1:871424/129‑1 (MQ=255)
                                                                                                                              gggACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGc    >  1:500501/1‑139 (MQ=255)
                                                                                                                                gACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCAt  <  1:766708/139‑1 (MQ=255)
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CGCGAAGTTTTGGATAACGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCAT  >  NC_000913/936068‑936334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑