Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,036,083	C→T	A105V (GCG→GTG)	hyaD →	hydrogenase 1 maturation protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,036,083	0	C	T	100.0%	116.4 / NA	34	A105V (GCG→GTG)	hyaD	hydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

TCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCG  >  NC_000913/1035958‑1036216                                                                                                                              |                                                                                                                                      tctcGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATc                                                                                                                          >  1:211843/1‑139 (MQ=255)    cGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCcgc                                                                                                                       >  2:750812/1‑139 (MQ=255)      aTGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCgg                                                                                                                     >  1:1082005/1‑139 (MQ=255)      aTGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCgg                                                                                                                     >  1:672012/1‑139 (MQ=255)         ccATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACTCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGAGGATATCCGCGGACa                                                                                                                  <  1:740781/139‑1 (MQ=255)             tGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTg                                                                                                              >  2:1047983/1‑139 (MQ=255)                 tACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                          >  1:845977/1‑139 (MQ=255)                 tACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                          >  1:94885/1‑139 (MQ=255)                 tACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                          <  2:1023246/139‑1 (MQ=255)                  aCGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGc                                                                                                         <  2:211843/139‑1 (MQ=255)                   cGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGca                                                                                                        >  2:356893/1‑139 (MQ=255)                      gCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACat                                                                                                     >  2:803087/1‑139 (MQ=255)                         ggaacctggaacGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                          <  1:37362/131‑1 (MQ=255)                         ggaacctggaacGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                          >  2:37362/1‑131 (MQ=255)                         ggaacctggaacGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATAtt                                                                                                  >  2:372944/1‑139 (MQ=255)                                ggaacGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcg                                                                                           >  1:468410/1‑139 (MQ=255)                                       tGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCt                                                                                    <  1:803087/139‑1 (MQ=255)                                               tATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCg                                                                            <  1:821346/139‑1 (MQ=255)                                                          aCGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgac                                                                 <  1:1047983/139‑1 (MQ=255)                                                           cGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgacg                                                                >  1:506781/1‑139 (MQ=255)                                                           cGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgacg                                                                >  2:523390/1‑139 (MQ=255)                                                               ttCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTa                                                            <  2:802477/139‑1 (MQ=255)                                                               ttCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTa                                                            <  2:1089810/139‑1 (MQ=255)                                                               ttCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTACAACCCGCAATGCTCGACGACTa                                                            <  1:356893/139‑1 (MQ=255)                                                                      ttATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGt                                                     <  2:249213/139‑1 (MQ=255)                                                                        aTCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAg                                                   >  2:847021/1‑139 (MQ=255)                                                                              gcgcGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAg                                             >  1:347432/1‑139 (MQ=255)                                                                                  gaagaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGaa                                         <  2:672012/139‑1 (MQ=255)                                                                                           gAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACgg                                <  2:468410/139‑1 (MQ=255)                                                                                            aGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACggg                               <  2:94885/139‑1 (MQ=255)                                                                                                                     aaGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAggc      <  2:347432/139‑1 (MQ=255)                                                                                                                       gtgtTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAggcgg    >  1:161462/1‑139 (MQ=255)                                                                                                                          ttGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGgcggcg  >  2:409108/1‑138 (MQ=255)                                                                                                                          ttGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGgcggcg  <  1:409108/138‑1 (MQ=255)                                                                                                                              |                                                                                                                                      TCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCG  >  NC_000913/1035958‑1036216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑