Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,077,868	G→A	R339C (CGC→TGC)	putA ←	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,077,868	0	G	A	100.0%	156.4 / NA	46	R339C (CGC→TGC)	putA	fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

ATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATAGGAAACCATATACGCCTGTGCAT  >  NC_000913/1077737‑1077996                                                                                                                                    |                                                                                                                                 aTCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATaa                                                                                                                           >  1:859992/1‑139 (MQ=255)           aTCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCa                                                                                                                 <  1:59529/139‑1 (MQ=255)            tctcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAg                                                                                                                <  1:277342/139‑1 (MQ=255)            tctcCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAg                                                                                                                >  2:737382/1‑139 (MQ=255)                cAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGCGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGcc                                                                                                            >  1:576944/1‑139 (MQ=255)                       tCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGtt                                                                                                     >  2:674278/1‑139 (MQ=255)                                 cGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAAt                                                                                           >  1:57919/1‑139 (MQ=255)                                    cGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        <  2:980815/139‑1 (MQ=255)                                    cGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        <  2:859992/139‑1 (MQ=255)                                      tCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                                                                                      <  2:839499/139‑1 (MQ=255)                                      tCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                                                                                      <  1:737382/139‑1 (MQ=255)                                       cAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     <  2:576944/139‑1 (MQ=255)                                         aTGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                                                                                      <  1:440810/136‑1 (MQ=255)                                         aTGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                                                                                      >  2:440810/1‑136 (MQ=255)                                         aTGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGcc                                                                                   >  2:258103/1‑139 (MQ=255)                                              gATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCa                                                                              <  1:258103/139‑1 (MQ=255)                                               aTACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCTATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCAt                                                                             >  2:296355/1‑139 (MQ=255)                                                     atatCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGa                                                                                                          <  2:835872/104‑1 (MQ=255)                                                     atatCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGa                                                                                                          >  1:835872/1‑104 (MQ=255)                                                      tatCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGcc                                                                      >  2:992270/1‑139 (MQ=255)                                                         cGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                                                                                      <  1:932948/120‑1 (MQ=255)                                                         cGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCg                                                                                      >  2:932948/1‑120 (MQ=255)                                                          gTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        >  2:621605/1‑117 (MQ=255)                                                          gTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        <  1:621605/117‑1 (MQ=255)                                                             cTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        >  1:760435/1‑114 (MQ=255)                                                             cTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        <  2:760435/114‑1 (MQ=255)                                                                aCGCGCCAGCGGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     <  1:955456/114‑1 (MQ=255)                                                                aCGCGCCAGCGGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     >  2:955456/1‑114 (MQ=255)                                                                aCGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAAGGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTa                                                            >  1:794563/1‑139 (MQ=255)                                                                aCGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        <  1:810175/111‑1 (MQ=255)                                                                aCGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGcc                                                                                        >  2:810175/1‑111 (MQ=255)                                                                aCGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     <  2:907927/114‑1 (MQ=255)                                                                aCGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     >  1:907927/1‑114 (MQ=255)                                                                     ccAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTa                                                            >  2:611502/1‑134 (MQ=255)                                                                     ccAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTa                                                            <  1:611502/134‑1 (MQ=255)                                                                            ggTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCg                                                <  2:57919/139‑1 (MQ=255)                                                                                agtgaTTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATgg                                            >  2:1084144/1‑139 (MQ=255)                                                                                      ttCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAg                                                                                                                >  1:347423/1‑65 (MQ=255)                                                                                      ttCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAg                                                                                                                <  2:347423/65‑1 (MQ=255)                                                                                                          ttCCATTACCCGGTCATAGTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATAGGAAAcc                  >  1:616881/1‑139 (MQ=255)                                                                                                           tCCATTACCCGGTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATAGGAAACCa                 >  2:293485/1‑139 (MQ=255)                                                                                                                      gTCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATAGGAAACCATATACGCCtgt      >  1:232829/1‑139 (MQ=255)                                                                                                                       tCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     >  1:391497/1‑59 (MQ=255)                                                                                                                       tCATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCgg                                                                                     <  2:391497/59‑1 (MQ=255)                                                                                                                        cATACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATGGGAAAGCATATACGCCTGTGc    >  1:645796/1‑139 (MQ=255)                                                                                                                          tACTGGGCGCAGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATAGGAAACCATATACGCCTGTGCAt  <  1:515370/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 ATCGAGGGAGATCTCCAGGCGATCGGACTCTTCGGCGTCAATGTTGATACCAATATCGTACTGACGCGCCAGCAGGGTGAGTGATTTCAGACGCGGGTAAAGCTCTTCCATTACCCGGTCATACTGGGCGCGGCTATAACGCGGATGCAGCGCCGACAGTTTGATTGAAATGCCCGGCCCTTCATAGATGCCACGACCGTTAGACGCTTTACCGATGGCGTGAATCGCCTGCTGATAGGAAACCATATACGCCTGTGCAT  >  NC_000913/1077737‑1077996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑