Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,337	G→A	R428C (CGC→TGC)	narX ←	sensory histidine kinase in two‑component regulatory system with NarL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,337	0	G	A	100.0%	162.9 / NA	47	R428C (CGC→TGC)	narX	sensory histidine kinase in two‑component regulatory system with NarL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

GTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTT  >  NC_000913/1276203‑1276471                                                                                                                                       |                                                                                                                                       gTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAgctg                                                                                                                                    >  1:885958/1‑139 (MQ=255) gTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAgctg                                                                                                                                    <  2:162418/139‑1 (MQ=255)           cAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCa                                                                                                                          <  1:180391/139‑1 (MQ=255)           cAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCa                                                                                                                          <  1:650309/139‑1 (MQ=255)            aaCTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAg                                                                                                                         >  2:238903/1‑139 (MQ=255)                   cTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCg                                                                                                                  >  1:370469/1‑139 (MQ=255)                        gcgcCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTg                                                                                                             <  1:378150/139‑1 (MQ=255)                        gcgcCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTg                                                                                                             <  2:584161/139‑1 (MQ=255)                                         ggCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCa                                                                                                                     >  1:297914/1‑114 (MQ=255)                                         ggCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCa                                                                                                                     <  2:297914/114‑1 (MQ=255)                                               gTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAgc                                                                                                                                      >  1:287412/1‑91 (MQ=255)                                               gTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCa                                                                                      >  1:1075320/1‑139 (MQ=255)                                               gTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCa                                                                                      >  1:250598/1‑139 (MQ=255)                                                tGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCAt                                                                                     <  2:250598/139‑1 (MQ=255)                                                       cAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATg                                                                              <  1:397978/139‑1 (MQ=255)                                                            ggAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAaga                                                                         <  2:1075320/139‑1 (MQ=255)                                                             gAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagag                                                                        <  2:376242/139‑1 (MQ=255)                                                              aaTGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCACCTGTAAACAACTCACCTGCATCTTCATGCAagaga                                                                       <  2:972184/139‑1 (MQ=255)                                                                     tgAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTg                                                                <  2:873581/139‑1 (MQ=255)                                                                     tgAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTg                                                                <  2:370469/139‑1 (MQ=255)                                                                       aGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACt                                                                                              <  2:970719/107‑1 (MQ=255)                                                                       aGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACt                                                                                              >  1:970719/1‑107 (MQ=255)                                                                       aGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTggg                                                              <  1:562972/139‑1 (MQ=255)                                                                            ttCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATa                                                         >  1:335283/1‑139 (MQ=255)                                                                            ttCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATa                                                         >  1:519262/1‑139 (MQ=255)                                                                                      tGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCa                                               >  2:885460/1‑139 (MQ=255)                                                                                        cgcCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCTATAGAATCATGCAGt                                             >  1:767111/1‑139 (MQ=255)                                                                                                        aGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAAtggtgg                             >  2:831893/1‑139 (MQ=255)                                                                                                         gTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAaac                                                                                                  <  1:846806/69‑1 (MQ=255)                                                                                                         gTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAaac                                                                                                  >  2:846806/1‑69 (MQ=255)                                                                                                           tCGTTACGGATCGGACTTAGCAGGGCGCAGTTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACGCACCTGCATCATCATGCAAGAGAGAGATTGTGCAATTGAATCATGCAGTTCGCGCGCAATGGTGGCAc                          <  1:460029/139‑1 (MQ=255)                                                                                                             gTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGc                        >  1:609612/1‑139 (MQ=255)                                                                                                               tACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGccc                                                                                                               >  2:982712/1‑50 (MQ=255)                                                                                                               tACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGccc                                                                                                               <  1:982712/50‑1 (MQ=255)                                                                                                                    aTCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCa                 >  1:733861/1‑139 (MQ=255)                                                                                                                      cTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATc               >  2:481669/1‑139 (MQ=255)                                                                                                                      cTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATc               >  2:1085184/1‑139 (MQ=255)                                                                                                                       tGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCa              >  2:69358/1‑139 (MQ=255)                                                                                                                        gACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTcg                                          >  2:756503/1‑110 (MQ=255)                                                                                                                        gACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTcg                                          <  1:756503/110‑1 (MQ=255)                                                                                                                         aCTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACACCTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGa                                                       >  1:1094624/1‑96 (MQ=255)                                                                                                                         aCTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGa                                                       <  2:1094624/96‑1 (MQ=255)                                                                                                                                   tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAgagagaga                                                                   >  1:666075/1‑74 (MQ=255)                                                                                                                                   tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAgagagaga                                                                   <  2:666075/74‑1 (MQ=255)                                                                                                                                   tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGtt  >  2:33821/1‑139 (MQ=255)                                                                                                                                   tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGtt  >  1:467382/1‑139 (MQ=255)                                                                                                                                   tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGtt  >  1:1007356/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       GTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAACTGTT  >  NC_000913/1276203‑1276471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑