Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,458,617 A→G A118A (GCA→GCG paaG → 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,6170AG100.0% 91.3 / NA 25A118A (GCA→GCGpaaG1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/12);  total (13/12)

AGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCG  >  NC_000913/1458485‑1458741
                                                                                                                                    |                                                                                                                            
aGGCCCCGCACGGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTgggg                                                                                                                        >  1:644628/1‑139 (MQ=255)
     ccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                                                                   <  2:644628/139‑1 (MQ=255)
       gCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACa                                                                                                                 >  2:807925/1‑139 (MQ=255)
                         tCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGtt                                                                                               <  1:855756/139‑1 (MQ=255)
                             ttGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGc                                                                                           >  1:623861/1‑139 (MQ=255)
                                                 cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                       <  2:623861/139‑1 (MQ=255)
                                                   ggTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCATCATGGCGTTTAGt                                                                     <  2:705168/139‑1 (MQ=255)
                                                       cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                                                                 >  2:1090427/1‑139 (MQ=255)
                                                       cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTGATTGCCGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                                                                 <  2:742502/139‑1 (MQ=255)
                                                        gTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                <  1:1090427/139‑1 (MQ=255)
                                                            ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATtgctgc                                                                                                    >  2:740096/1‑99 (MQ=255)
                                                            ccTGGCAAAACAGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGCCATCGTTATtgctgc                                                                                                    <  1:740096/99‑1 (MQ=255)
                                                              tGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAgg                                                             <  1:196555/136‑1 (MQ=255)
                                                              tGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAgg                                                             >  2:196555/1‑136 (MQ=255)
                                                                                   tGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAAc                                     <  1:26999/139‑1 (MQ=255)
                                                                                               tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGcc                         >  2:889935/1‑139 (MQ=255)
                                                                                                 aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                <  1:749904/50‑1 (MQ=255)
                                                                                                 aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                >  2:749904/1‑50 (MQ=255)
                                                                                                  aTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAcg                      <  1:626837/139‑1 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGTGGTGGAACCTGGTTACTGCCAcgcg                    >  2:856131/1‑139 (MQ=255)
                                                                                                    ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAcgcg                    >  1:893476/1‑139 (MQ=255)
                                                                                                      cgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCCGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATAccc                                                   <  2:725606/106‑1 (MQ=255)
                                                                                                      cgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTAAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATAccc                                                   >  1:725606/1‑106 (MQ=255)
                                                                                                         ggcggcAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGcc               >  1:658408/1‑139 (MQ=255)
                                                                                                                      ggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcgcg  >  1:401464/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                            
AGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCG  >  NC_000913/1458485‑1458741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: