Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,702,700	T→C	F156F (TTT→TTC)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,702,700	0	T	C	100.0%	133.4 / NA	36	F156F (TTT→TTC)	add	adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

TAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTTTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCG  >  NC_000913/1702567‑1702830                                                                                                                                      |                                                                                                                                   tAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               >  2:541167/1‑139 (MQ=255) tAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               <  1:541167/139‑1 (MQ=255) tAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               <  1:72979/139‑1 (MQ=255)    cGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCa                                                                                                                            <  2:41723/139‑1 (MQ=255)        tgttgtCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGt                                                                                                                        >  2:876668/1‑139 (MQ=255)           tgtCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGAc                                                                                                                     <  1:236649/139‑1 (MQ=255)           tgtCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGAc                                                                                                                     >  2:938196/1‑139 (MQ=255)                    ggTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTAcc                                                                                                            >  2:635635/1‑139 (MQ=255)                       gATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCa                                                                                                         >  2:336223/1‑139 (MQ=255)                                cGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTa                                                                                                >  1:517551/1‑139 (MQ=255)                                  tACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               >  1:231867/1‑106 (MQ=255)                                  tACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               <  2:231867/106‑1 (MQ=255)                                  tACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGc                                                                                              >  2:63200/1‑139 (MQ=255)                                    cGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCCGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCg                                                                                            >  1:301239/1‑139 (MQ=255)                                               ccGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACtt                                                                                 >  1:1107731/1‑139 (MQ=255)                                                         ggTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCgg                                                                       >  1:846391/1‑139 (MQ=255)                                                         ggTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCgg                                                                       <  1:635635/139‑1 (MQ=255)                                                           tgtgCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGa                                                                     <  1:876668/139‑1 (MQ=255)                                                              gCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGt                                                                  <  2:243740/139‑1 (MQ=255)                                                               cAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTc                                                                 >  2:506850/1‑139 (MQ=255)                                                                aGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCt                                                                <  1:274071/139‑1 (MQ=255)                                                                     aaGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTcc                                                           >  1:994673/1‑139 (MQ=255)                                                                       gCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGtg                                                                                         <  2:1110240/107‑1 (MQ=255)                                                                       gCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGtg                                                                                         >  1:1110240/1‑107 (MQ=255)                                                                        cTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCttt                                                        <  2:846391/139‑1 (MQ=255)                                                                          tATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTtct                                                      <  2:994673/139‑1 (MQ=255)                                                                                  ttATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCaa                                              >  2:941333/1‑139 (MQ=255)                                                                                  ttATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATGTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTGTCACTTCaa                                              >  1:400989/1‑139 (MQ=255)                                                                                   tATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAAc                                             >  2:42194/1‑139 (MQ=255)                                                                                             aCCTTCGACGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTg                                   >  1:454757/1‑139 (MQ=255)                                                                                                     cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  2:20639/139‑1 (MQ=255)                                                                                                     cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  2:1107731/139‑1 (MQ=255)                                                                                                     cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  1:739085/139‑1 (MQ=255)                                                                                                     cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  1:506850/139‑1 (MQ=255)                                                                                                         gccgccTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGc                       <  2:517551/139‑1 (MQ=255)                                                                                                                              gAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCg  >  1:1034519/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                   TAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTTTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCG  >  NC_000913/1702567‑1702830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑