Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,722,854	T→C	L245P (CTC→CCC)	ydhK →	putative efflux protein (PET) component of YdhJK efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,722,854	0	T	C	100.0%	117.9 / NA	33	L245P (CTC→CCC)	ydhK	putative efflux protein (PET) component of YdhJK efflux pump
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

GAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCGAACAGTTGCTGACGGCGCTCGCCAGTTCGCAAACA  >  NC_000913/1722721‑1722984                                                                                                                                      |                                                                                                                                   gAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCccacc                                                                                                                               <  1:634843/139‑1 (MQ=255)   aacaacCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAg                                                                                                                             <  1:826816/139‑1 (MQ=255)       acCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAAt                                                                                                                         <  1:511793/139‑1 (MQ=255)        ccGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAg                                                                                                                             <  1:763313/134‑1 (MQ=255)        ccGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAg                                                                                                                             >  2:763313/1‑134 (MQ=255)            tGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGt                                                                                                                    >  1:862718/1‑139 (MQ=255)             gCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCccacc                                                                                                                               <  2:713547/127‑1 (MQ=255)             gCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCccacc                                                                                                                               >  1:713547/1‑127 (MQ=255)                         gcaCATGAAGGGTTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCCCTGCCCCACCAGCAATTACGTATGACCAGTCTCa                                                                                                       >  1:542056/1‑139 (MQ=255)                                    ggTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGt                                                                                                                    >  1:508420/1‑115 (MQ=255)                                    ggTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGt                                                                                                                    <  2:508420/115‑1 (MQ=255)                                    ggTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTg                                                                                            >  2:123871/1‑139 (MQ=255)                                    ggTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTg                                                                                            >  2:1029906/1‑139 (MQ=255)                                      tGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg                                                                                          <  1:606068/139‑1 (MQ=255)                                        aTTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGAc                                                                                        <  2:284200/139‑1 (MQ=255)                                                       aCCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCccacca                                                                                                                              >  1:510848/1‑86 (MQ=255)                                                       aCCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCccacca                                                                                                                              <  2:510848/86‑1 (MQ=255)                                                       aCCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACt                                                                         >  1:725402/1‑139 (MQ=255)                                                          aTGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGc                                                                      <  2:587097/139‑1 (MQ=255)                                                             aaTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCt                                                                   <  1:1029906/139‑1 (MQ=255)                                                                 tgctgcGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGTCTg                                                                                            >  2:41583/1‑110 (MQ=255)                                                                 tgctgcGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCAcc                                                               >  2:76875/1‑139 (MQ=255)                                                                 tgctgcGTATCCAGGCGTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGTCTg                                                                                            <  1:41583/110‑1 (MQ=255)                                                                   ctgcGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCATCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGc                                                             <  1:666348/139‑1 (MQ=255)                                                                              ggCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCaa                                                                                                                          >  1:529023/1‑67 (MQ=255)                                                                              ggCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCaa                                                                                                                          <  2:529023/67‑1 (MQ=255)                                                                                           cACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCg                                     <  2:1005678/139‑1 (MQ=255)                                                                                                       cGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGc                                                                      <  2:165272/94‑1 (MQ=255)                                                                                                       cGCCAGCAAAACGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGc                                                                      >  1:165272/1‑94 (MQ=255)                                                                                                                 aCGCGCGCCTTAATGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCGAACAGTTGCTGACGGCGCTCGc               <  2:928918/139‑1 (MQ=255)                                                                                                                            aaTGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCGAACAGTTGCTGACGGCGCTCGCCAGTTCGCaaa    <  1:72037/139‑1 (MQ=255)                                                                                                                              tGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCGAACAGTTGCTGACGGCGCTCGCCAGTTCGCAAACa  <  1:260916/139‑1 (MQ=255)                                                                                                                              tGCGCTGCCCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCGAACAGTTGCTGACGGCGCTCGCCAGTTCGCAAACa  <  1:123871/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   GAAACAACCGATGCCATTCGTGCAGCACATGAAGGGGTGATTGGGCAGATACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACGTATGTTGCTCAACTGGCCCTCACCGCCAGGTGCCACACGAGAAATTCTCGAACAGTTGCTGACGGCGCTCGCCAGTTCGCAAACA  >  NC_000913/1722721‑1722984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑