Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,320	G→A	R14C (CGC→TGC)	uvrY ←	response regulator in two‑component regulatory system with BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,320	0	G	A	100.0%	207.9 / NA	60	R14C (CGC→TGC)	uvrY	response regulator in two‑component regulatory system with BarA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/31);  total (29/31)

ATGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCGCACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAA  >  NC_000913/1995196‑1995447                                                                                                                             |                                                                                                                                aTGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt                                                                                                                   <  2:234394/139‑1 (MQ=255)  tGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGtc                                                                                                                  >  2:1100080/1‑139 (MQ=255)    tCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGtcat                                                                                                                >  1:666124/1‑139 (MQ=255)        ttAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATGCCTGCGCACGCCAGTTCGTGGTCATcaac                                                                                                            <  1:100711/139‑1 (MQ=255)        ttAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                            <  1:139023/139‑1 (MQ=255)         tAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaaca                                                                                                           >  1:268089/1‑139 (MQ=255)             accacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTa                                                                                                       <  2:444699/139‑1 (MQ=255)             accacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTa                                                                                                       >  2:352991/1‑139 (MQ=255)              ccacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAg                                                                                                      >  2:306815/1‑139 (MQ=255)                  gTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCGCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACg                                                                                                  >  1:249604/1‑139 (MQ=255)                        ggCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCGCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATc                                                                                            <  2:249604/139‑1 (MQ=255)                         gCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCa                                                                                           <  1:368708/139‑1 (MQ=255)                                     caccacTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATAtctc                                                                               <  2:268089/139‑1 (MQ=255)                                        cacTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAg                                                                            >  2:703267/1‑139 (MQ=255)                                          cTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                            >  1:1090414/1‑105 (MQ=255)                                          cTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                            <  2:1090414/105‑1 (MQ=255)                                          cTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGaa                                                                          >  1:844536/1‑139 (MQ=255)                                                 gCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGa                                                                   <  1:352991/139‑1 (MQ=255)                                                  cGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGAt                                                                  >  1:238801/1‑139 (MQ=255)                                                    tCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATaa                                                                >  2:502941/1‑139 (MQ=255)                                                    tCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATaa                                                                <  2:254917/139‑1 (MQ=255)                                                    tCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATaa                                                                >  1:526910/1‑139 (MQ=255)                                                    tCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATaa                                                                >  1:92946/1‑139 (MQ=255)                                                      ttCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACg                                                              <  1:1100080/139‑1 (MQ=255)                                                       tCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGc                                                             <  2:4678/139‑1 (MQ=255)                                                         aCCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTa                                                           <  2:666124/139‑1 (MQ=255)                                                           cGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt                                                         <  2:800420/139‑1 (MQ=255)                                                           cGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt                                                         >  2:655989/1‑139 (MQ=255)                                                            gCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTg                                                        >  1:346324/1‑139 (MQ=255)                                                                    ccTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttag                                                <  1:703267/139‑1 (MQ=255)                                                                        aCCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt                                            <  2:238801/139‑1 (MQ=255)                                                                        aCCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt                                            <  1:1002607/139‑1 (MQ=255)                                                                          cgacgaCTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTc                                          <  1:502941/139‑1 (MQ=255)                                                                            acgacTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCacac                                                                                                                              >  1:258239/1‑53 (MQ=255)                                                                            acgacTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCacac                                                                                                                              <  2:258239/53‑1 (MQ=255)                                                                            acgacTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTg                                                                                                                     <  1:409722/62‑1 (MQ=255)                                                                            acgacTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTg                                                                                                                     >  2:409722/1‑62 (MQ=255)                                                                              gacTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCCGTTCGTGGTCATCAACAAg                                                                                                         >  1:456842/1‑72 (MQ=255)                                                                              gacTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAg                                                                                                         <  2:456842/72‑1 (MQ=255)                                                                                cTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTatat                                    <  1:1646/139‑1 (MQ=255)                                                                                    tataCCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGc                                >  1:1063861/1‑139 (MQ=255)                                                                                          cTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGtttt                          <  1:306110/139‑1 (MQ=255)                                                                                           tttATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtta                                                 >  2:515210/1‑115 (MQ=255)                                                                                           tttATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtta                                                 <  1:515210/115‑1 (MQ=255)                                                                                             tataTCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCaa                                                                                          >  2:394306/1‑72 (MQ=255)                                                                                             tataTCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCaa                                                                                          <  1:394306/72‑1 (MQ=255)                                                                                             tataTCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGAt                                                                  >  2:958965/1‑96 (MQ=255)                                                                                             tataTCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGAt                                                                  <  1:958965/96‑1 (MQ=255)                                                                                                atCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGc                    <  2:844536/139‑1 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAACGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAAc                 >  1:559358/1‑139 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt                                                         <  2:454520/99‑1 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt                                                         >  1:454520/1‑99 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAAc                 >  2:136144/1‑139 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACtt               >  1:822431/1‑139 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACtt               >  2:634618/1‑139 (MQ=255)                                                                                                      cAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCaa                                                                                          <  1:556725/63‑1 (MQ=255)                                                                                                      cAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCaa                                                                                          >  2:556725/1‑63 (MQ=255)                                                                                                        gAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGa            <  1:29402/139‑1 (MQ=255)                                                                                                           tGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAAc         >  1:523391/1‑139 (MQ=255)                                                                                                             cgtcgtATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGt       >  1:1028854/1‑139 (MQ=255)                                                                                                                cgtATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTaa    <  1:310064/139‑1 (MQ=255)                                                                                                                  tATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTaaaa  <  2:822431/139‑1 (MQ=255)                                                                                                                         gcgcacACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttagt                                               <  2:199605/87‑1 (MQ=255)                                                                                                                         gcgcacACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttagt                                               >  1:199605/1‑87 (MQ=255)                                                                                                                          cgcacACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAat                                                                                    <  2:552984/49‑1 (MQ=255)                                                                                                                             |                                                                                                                                ATGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCGCACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAA  >  NC_000913/1995196‑1995447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑