Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,073,094 T→C L519P (CTG→CCG)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,073,0940TC99.3% 564.5 / NA 146L519P (CTG→CCG) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (65/80);  minor base A (1/0);  total (66/80)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAA  >  NC_000913/2072958‑2073229
                                                                                                                                        |                                                                                                                                       
aTCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                       >  1:812005/1‑139 (MQ=255)
 tCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgac                                                                                                                                      >  1:484044/1‑139 (MQ=255)
 tCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgac                                                                                                                                      >  2:634580/1‑139 (MQ=255)
 tCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgac                                                                                                                                      >  2:361543/1‑139 (MQ=255)
  ccGTGAAGGCGATGCACTCCTGCATGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                     >  1:750671/1‑139 (MQ=255)
   cGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgc                                                                                                                                    >  1:684315/1‑139 (MQ=255)
     tGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  <  1:558133/139‑1 (MQ=255)
     tGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  >  2:1085852/1‑139 (MQ=255)
      gAAGGCGATGCACTCCTGCAGGGAGGCTCACTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCAcgccgacgctga                                                                                                                                 <  1:716962/139‑1 (MQ=255)
          gCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGa                                                                                                                             <  2:304004/139‑1 (MQ=255)
               gCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTa                                                                                                                        >  2:954676/1‑139 (MQ=255)
                cACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGGCAGTAc                                                                                                                       <  2:820133/139‑1 (MQ=255)
                   tCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGt                                                                                                                    <  1:414785/139‑1 (MQ=255)
                     cTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTca                                                                                                                  <  2:276411/139‑1 (MQ=255)
                      tGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcac                                                                                                                 <  1:475383/139‑1 (MQ=255)
                       gCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                                <  2:975758/139‑1 (MQ=255)
                       gCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                                <  1:672874/139‑1 (MQ=255)
                        cAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacca                                                                                                               >  2:982643/1‑139 (MQ=255)
                          gggAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACg                                                                                                             <  2:535829/139‑1 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  <  2:889019/115‑1 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  >  1:889019/1‑115 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                          >  2:365320/1‑139 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                          >  1:185559/1‑139 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGAt                                                                                                          >  1:899843/1‑139 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATg                                                                                                         >  1:811371/1‑139 (MQ=255)
                                 tctctcACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCa                                                                                                      <  2:522068/139‑1 (MQ=255)
                                      cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                 <  2:800570/139‑1 (MQ=255)
                                        ccGGTAACGGCAGCGTGGAAAAATCAGGAACTGGCACACTCACTGTCAGTAACACCACTCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACTCCGACGCTCAACGCCAGTACCGTCACCACGGATGTCATTGCTCa                                                                                               <  1:377334/139‑1 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            >  2:138034/1‑139 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  1:310910/139‑1 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTCGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  2:750671/139‑1 (MQ=255)
                                            tAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            >  2:928313/1‑138 (MQ=255)
                                            tAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  1:928313/138‑1 (MQ=255)
                                             aaCGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                                >  1:444717/1‑117 (MQ=255)
                                             aaCGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGcgcg                                                                                          >  2:734005/1‑139 (MQ=255)
                                               cGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGt                                                                                        >  2:244748/1‑139 (MQ=255)
                                                ggCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTa                                                                                       <  1:544483/139‑1 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           >  2:120549/1‑114 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           <  1:120549/114‑1 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                                                  >  2:450436/1‑139 (MQ=255)
                                                       tGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCt                                                                                >  1:232075/1‑139 (MQ=255)
                                                            aaaTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGc                                                                           <  1:876857/139‑1 (MQ=255)
                                                              aTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                         <  2:484044/139‑1 (MQ=255)
                                                               tCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  1:924055/119‑1 (MQ=255)
                                                               tCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            >  2:924055/1‑119 (MQ=255)
                                                                   gAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCgg                                                                    <  2:185559/139‑1 (MQ=255)
                                                                   gAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCgg                                                                    <  2:967336/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  1:203737/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  2:17147/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  2:1066455/1‑139 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  1:95822/139‑1 (MQ=255)
                                                                         gCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCAc                                                              >  1:1020297/1‑139 (MQ=255)
                                                                         gCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCAc                                                              >  1:98001/1‑139 (MQ=255)
                                                                                 actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                       <  2:417927/58‑1 (MQ=255)
                                                                                 actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            >  1:712437/1‑101 (MQ=255)
                                                                                 actGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  2:712437/101‑1 (MQ=255)
                                                                                  ctGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGaa                                                     <  1:734005/139‑1 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAAc                                                    >  2:385778/1‑139 (MQ=255)
                                                                                     tCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACgg                                                  <  2:176294/139‑1 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  1:77010/93‑1 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                                      <  2:1003569/99‑1 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                                      >  1:1003569/1‑99 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  2:594925/1‑122 (MQ=255)
                                                                                           acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAt                                            <  2:502859/139‑1 (MQ=255)
                                                                                               caccCTCACCCAGCAAGCCGTCAACCTTAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAc                                        <  1:128189/139‑1 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacc                                                                                                                >  1:273555/1‑67 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAACACTGTGCTGAACGGTGCCATTGAc                                        >  2:1014132/1‑139 (MQ=255)
                                                                                                 cacTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                                <  1:1050183/49‑1 (MQ=255)
                                                                                                 cacTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                                >  2:1050183/1‑49 (MQ=255)
                                                                                                  acTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAcccc                                     <  1:365320/139‑1 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                                      <  2:754837/86‑1 (MQ=255)
                                                                                                       cccAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  >  1:4521/1‑41 (MQ=255)
                                                                                                       cccAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  <  2:4521/41‑1 (MQ=255)
                                                                                                       cccAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGaa                                >  1:668946/1‑139 (MQ=255)
                                                                                                           gAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCgg                                                                                         >  2:257735/1‑78 (MQ=255)
                                                                                                           gAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCgg                                                                                         <  1:257735/78‑1 (MQ=255)
                                                                                                            aaaGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCa                           >  1:794295/1‑139 (MQ=255)
                                                                                                                ccGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCActct                       >  1:416521/1‑139 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  1:331691/97‑1 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  2:331691/1‑97 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                     <  1:244748/139‑1 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                     >  1:386911/1‑139 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                     >  1:406015/1‑139 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                     >  1:495493/1‑139 (MQ=255)
                                                                                                                        cTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCg               <  2:24803/139‑1 (MQ=255)
                                                                                                                          gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                           >  2:98804/1‑109 (MQ=255)
                                                                                                                          gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                           <  1:98804/109‑1 (MQ=255)
                                                                                                                          gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt             <  2:271169/139‑1 (MQ=255)
                                                                                                                          gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt             <  1:629768/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            <  2:668946/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            <  2:808505/139‑1 (MQ=255)
                                                                                                                            atgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGc           <  1:176920/139‑1 (MQ=255)
                                                                                                                             tgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGcc          >  1:139505/1‑139 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCAcc       <  2:595757/139‑1 (MQ=255)
                                                                                                                                 ggCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCt      >  1:549945/1‑139 (MQ=255)
                                                                                                                                    acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa   <  2:495493/139‑1 (MQ=255)
                                                                                                                                     cgccgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            >  2:554004/1‑129 (MQ=255)
                                                                                                                                     cgccgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCAcc       >  1:35066/1‑134 (MQ=255)
                                                                                                                                     cgccgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGaa  >  2:770516/1‑139 (MQ=255)
                                                                                                                                     cgccgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGaa  >  1:702599/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAA  >  NC_000913/2072958‑2073229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: