Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,291	C→T	A331V (GCG→GTG)	yejF →	microcin C ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,291	0	C	T	100.0%	145.0 / NA	42	A331V (GCG→GTG)	yejF	microcin C ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/21);  total (21/21)

CATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAA  >  NC_000913/2277157‑2277413                                                                                                                                       |                                                                                                                           cATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTgctg                                                                                                                        >  2:702000/1‑139 (MQ=255)  aTTCGCAAAGGGCTCTTGAAGCGCCGTGTGGATCATAATTTGGTGGTGAGAAGCATCAGTTTTACGCTACGAGCGGCTGAAACGGTGGGTTTCGAGGGCAAGTCCGGTTCCGGGACAACTACGACGGGAGTGGTgctgt                                                                                                                       >  1:489999/1‑138 (MQ=255)    tcacAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGctgctg                                                                                                                     >  2:816653/4‑139 (MQ=255)    tCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGctgctg                                                                                                                     >  2:374775/1‑139 (MQ=255)       cAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGa                                                                                                                  <  1:935036/139‑1 (MQ=255)       cAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGa                                                                                                                  <  2:2584/139‑1 (MQ=255)         aaGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACt                                                                                                                <  2:789524/139‑1 (MQ=255)            ggATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGAt                                                                                                             <  1:816653/139‑1 (MQ=255)                      gcgcATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGG‑AAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAgg                                                                                                  >  2:68088/1‑139 (MQ=255)                        gcATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAggg                                                                                                 <  1:94175/139‑1 (MQ=255)                                      aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTg                                                                                   >  2:596139/1‑139 (MQ=255)                                            gtggtgAAAAACATCAGTTTTACGCTACGAGTGGGTGAAACAGTGGGTTTAGTGGGCGAGTCTGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATGCTCAGGGCAGCATCATCTTTGACGGTc                                                                             >  1:82761/1‑139 (MQ=255)                                            gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTc                                                                             >  1:824863/1‑139 (MQ=255)                                             tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  1:43899/1‑139 (MQ=255)                                             tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  2:850654/1‑139 (MQ=255)                                             tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  2:981654/1‑139 (MQ=255)                                             tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  1:270360/1‑139 (MQ=255)                                              ggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAg                                                                           >  2:999743/1‑139 (MQ=255)                                               gtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAGGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc                                                                          <  1:654649/139‑1 (MQ=255)                                                                      aCGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc                                                   <  1:293207/139‑1 (MQ=255)                                                                      aCGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGG‑AAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCgccgcc                                                  <  1:68088/139‑1 (MQ=255)                                                                       cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTgctg                                                                                                                        <  2:508160/69‑1 (MQ=255)                                                                       cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTgctg                                                                                                                        >  1:508160/1‑69 (MQ=255)                                                                       cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCgccgcc                                                  <  1:999743/139‑1 (MQ=255)                                                                           cGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCt                                              <  2:43899/139‑1 (MQ=255)                                                                              gTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGtt                                           <  2:270360/139‑1 (MQ=255)                                                                               tGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTa                                          >  1:445417/1‑139 (MQ=255)                                                                                         ggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATGGCCGCCAGCTGTTACCTATTCGTc                                >  2:619600/1‑139 (MQ=255)                                                                                         ggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTc                                >  2:805449/1‑139 (MQ=255)                                                                                          gTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCa                               <  1:981654/139‑1 (MQ=255)                                                                                          gTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCa                               <  1:374775/139‑1 (MQ=255)                                                                                           tttAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCAt                              <  2:82761/139‑1 (MQ=255)                                                                                                 gggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCAtt                        <  1:932461/139‑1 (MQ=255)                                                                                                   gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa                      >  2:65475/1‑139 (MQ=255)                                                                                                   gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa                      >  2:588572/1‑139 (MQ=255)                                                                                                     gAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAgg                    <  1:805449/139‑1 (MQ=255)                                                                                                     gAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAgg                    <  2:824863/139‑1 (MQ=255)                                                                                                      aGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAggt                   <  1:588572/139‑1 (MQ=255)                                                                                                              tCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTc           <  2:77201/139‑1 (MQ=255)                                                                                                                 gggAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAgg        >  2:578787/1‑139 (MQ=255)                                                                                                                 gggAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAg         <  2:21066/138‑1 (MQ=255)                                                                                                                 gggAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAg         >  1:21066/1‑138 (MQ=255)                                                                                                                       aGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCaa  >  1:354279/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                           CATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAA  >  NC_000913/2277157‑2277413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑