Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,314,922	T→C	S479P (TCC→CCC)	rcsD →	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,314,922	0	T	C	100.0%	85.1 / NA	24	S479P (TCC→CCC)	rcsD	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

TTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATG  >  NC_000913/2314791‑2315039                                                                                                                                    |                                                                                                                      ttCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGc                                                                                                                >  1:104229/1‑139 (MQ=255)       aTCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGagag                                                                                                          <  1:328387/139‑1 (MQ=255)                              aaCAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCgg                                                                                   >  2:124264/1‑139 (MQ=255)                                 aaGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGaaa                                                                                >  2:75719/1‑139 (MQ=255)                                                 cgcAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGaa                                                                >  2:148501/1‑139 (MQ=255)                                                   cAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATc                                                              <  2:1038919/139‑1 (MQ=255)                                                    aGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCa                                                             <  1:124264/139‑1 (MQ=255)                                                     gCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACTACTGGCGAATCAg                                                            <  1:959310/139‑1 (MQ=255)                                                       gTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGc                                                          <  1:148501/139‑1 (MQ=255)                                                        tCTGTATGAGAAAAACCAGCAGGGGCGGAAGATCTTTGTGAAATACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTATACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCa                                                         >  2:741510/1‑139 (MQ=255)                                                                  aaaaaCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTgg                                               >  2:857603/1‑139 (MQ=255)                                                                      aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg                                           >  1:207219/1‑139 (MQ=255)                                                                              gggCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtg                                   <  2:104229/139‑1 (MQ=255)                                                                              gggCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtg                                   <  1:16739/139‑1 (MQ=255)                                                                                   gatgatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaa                              >  2:541677/1‑139 (MQ=255)                                                                                    atgatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa                             <  2:964261/139‑1 (MQ=255)                                                                                       atCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATAc                          <  1:527392/139‑1 (MQ=255)                                                                                             aTGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt                                     <  1:162089/122‑1 (MQ=255)                                                                                             aTGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt                                     >  2:162089/1‑122 (MQ=255)                                                                                                   aaCATTGGAGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACTGTTAGCGAACa              >  2:721691/1‑139 (MQ=255)                                                                                                               gcgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg  >  1:450101/1‑139 (MQ=255)                                                                                                               gcgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg  <  1:857603/139‑1 (MQ=255)                                                                                                                cgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa                             <  1:999912/111‑1 (MQ=255)                                                                                                                cgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa                             >  2:999912/1‑111 (MQ=255)                                                                                                                                    |                                                                                                                      TTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATG  >  NC_000913/2314791‑2315039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑