| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,631,531 | G→A | G335G (GGC→GGT) | guaA ← | GMP synthetase (glutamine aminotransferase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,631,531 | 0 | G | A | 96.4% | 87.8 / ‑4.1 | 28 | G335G (GGC→GGT) | guaA | GMP synthetase (glutamine aminotransferase) |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/16); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAG > NC_000913/2631395‑2631657 | tGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTAcc < 1:358792/139‑1 (MQ=255) ctctTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCgcc > 1:297172/1‑139 (MQ=255) tCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCgccagcca > 1:21939/1‑139 (MQ=255) cGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTc < 2:21939/139‑1 (MQ=255) ggTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCa > 1:80704/1‑139 (MQ=255) gTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCAc < 2:970571/139‑1 (MQ=255) ttCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACg < 2:744379/139‑1 (MQ=255) tCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGt < 1:664131/139‑1 (MQ=255) tCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAg < 2:297172/139‑1 (MQ=255) aTCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGctt < 1:154635/139‑1 (MQ=255) ctctTTCGTCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttct < 1:851910/139‑1 (MQ=255) ttCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTc > 1:349577/1‑126 (MQ=255) ttCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTc < 2:349577/126‑1 (MQ=255) tCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATc > 2:315919/1‑139 (MQ=255) cGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCg < 2:12138/139‑1 (MQ=255) ccgccCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACtt < 2:80704/139‑1 (MQ=255) gtgAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGa > 2:817237/1‑139 (MQ=255) tgatgaCGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCgatgat > 2:1030471/1‑139 (MQ=255) gtgtGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGt > 1:1064659/1‑139 (MQ=255) cTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGc > 1:787768/1‑139 (MQ=255) aCCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTcc > 2:744889/1‑139 (MQ=255) tGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttct < 2:784444/82‑1 (MQ=255) tGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTACGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttct > 1:784444/1‑82 (MQ=255) gcAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCgccag < 1:95260/139‑1 (MQ=255) cgATTCGATAACGTCAGGGTAGATTGTACCCTGCGCCAGCCACCTCACGTCTTCCAGTTTCAGTGCTTCTTCATCGAATACTTCAACGAGAACGCGACCGATTTTTTTACGTTTTGCTTCCGGATCGTTTTCgccagcc < 1:1058600/138‑1 (MQ=255) aGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAg < 1:817237/139‑1 (MQ=255) aTGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCg > 2:550101/1‑78 (MQ=255) aTGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCg < 1:550101/78‑1 (MQ=255) | TGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAG > NC_000913/2631395‑2631657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |