Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,771,806 | A→T | intergenic (+191/+196) | yfjT → / ← yfjU | CP4‑57 prophage, putative periplasmic protein/CP4‑57 prophage, conserved protein,Phage or Prophage Related |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,771,806 | 0 | A | T | 96.5% | 196.0 / ‑0.8 | 58 | intergenic (+191/+196) | yfjT/yfjU | CP4‑57 prophage, putative periplasmic protein/CP4‑57 prophage, conserved protein,Phage or Prophage Related |
Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (24/32); total (25/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 |
TTCTCCTTAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGATCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAAC > NC_000913/2771676‑2771923 | ttCTCCTTAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACtttt < 2:435849/139‑1 (MQ=255) tctcCTTAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACttttt > 1:891223/1‑139 (MQ=255) cTTAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTc > 2:421719/1‑139 (MQ=255) tAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTa < 1:906098/139‑1 (MQ=255) ctcCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCATTTTTTGGTCTAAGCg < 1:740748/139‑1 (MQ=255) ctcCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg < 2:935379/139‑1 (MQ=255) ctcCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg < 2:891223/139‑1 (MQ=255) gTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTc < 2:863977/139‑1 (MQ=255) ggCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACttt > 2:102784/1‑116 (MQ=255) ggCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACttt < 1:102784/116‑1 (MQ=255) ggCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGt < 2:1088564/133‑1 (MQ=255) ggCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGt > 1:1088564/1‑133 (MQ=255) ggCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCata > 1:126428/1‑139 (MQ=255) ccACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATg < 2:126428/139‑1 (MQ=255) cGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTc < 1:370050/131‑1 (MQ=255) cGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTc > 2:370050/1‑131 (MQ=255) tACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCaa < 1:421719/139‑1 (MQ=255) aTGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATa < 1:117949/139‑1 (MQ=255) aGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAAc > 2:1078526/1‑139 (MQ=255) aGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGATCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAAc > 1:426468/1‑139 (MQ=255) tGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATgag > 2:425382/1‑139 (MQ=255) tGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATgag > 1:440545/1‑139 (MQ=255) tGGTCAGTTTATTACAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATgag > 1:743689/1‑139 (MQ=255) ggTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGt < 2:280232/91‑1 (MQ=255) ggTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGt > 1:280232/1‑91 (MQ=255) ggTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCat < 2:467288/108‑1 (MQ=255) ggTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCat > 1:467288/1‑108 (MQ=255) ggTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATgaga < 2:919683/139‑1 (MQ=255) tCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTTCTGTTCACTTTTTGGTCTAAGCg > 1:338431/1‑96 (MQ=255) tCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg < 2:338431/96‑1 (MQ=255) aGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGa > 2:885259/1‑139 (MQ=255) aGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGa < 2:241161/139‑1 (MQ=255) ttttttGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCAttaaaattaaaa < 1:363149/139‑1 (MQ=255) tttttGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTaaaaa > 2:519509/1‑139 (MQ=255) tttGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGATCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGa < 2:426468/139‑1 (MQ=255) tGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGata < 1:885259/139‑1 (MQ=255) tGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGata < 1:620110/139‑1 (MQ=255) tAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg > 1:92732/1‑59 (MQ=255) tAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg < 2:92732/59‑1 (MQ=255) cGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGt > 2:517796/1‑57 (MQ=255) cGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGt < 1:517796/57‑1 (MQ=255) gCTGTGAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGc < 1:1078526/139‑1 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCgtt < 1:748548/49‑3 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCgtt > 2:748548/1‑47 (MQ=38) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg > 1:840845/1‑47 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCg < 2:840845/47‑1 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGata > 2:504996/1‑121 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGata < 1:504996/121‑1 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATatgaat > 2:797886/1‑126 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATatgaat < 1:797886/126‑1 (MQ=255) tgAGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGat < 2:743689/139‑1 (MQ=255) aGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATgaga > 2:898611/1‑86 (MQ=255) aGCGCAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATgaga < 1:898611/86‑1 (MQ=255) cAGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAAc < 1:519509/139‑1 (MQ=255) aGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCata < 1:1099709/51‑1 (MQ=255) aGGCTGTGTTAAAACTCCTGTTCACTTTTTGGTCTAAGCGATGGTTTCata > 2:1099709/1‑51 (MQ=255) ccTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGata > 2:345340/1‑98 (MQ=255) ccTGTTCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGata < 1:345340/98‑1 (MQ=255) | TTCTCCTTAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGATCACTTTTTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAAC > NC_000913/2771676‑2771923 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |