Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,829,493	G→A	S149S (TCG→TCA)	srlR →	sorbitol‑inducible srl operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,829,493	0	G	A	100.0%	214.0 / NA	62	S149S (TCG→TCA)	srlR	sorbitol‑inducible srl operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/30);  total (32/30)

TCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCA  >  NC_000913/2829367‑2829628                                                                                                                               |                                                                                                                                        tCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             >  1:62131/1‑139 (MQ=255)       gCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  1:449574/139‑1 (MQ=255)        ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAAc                                                                                                                      <  1:816676/139‑1 (MQ=255)        ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAAc                                                                                                                      >  1:1046337/1‑139 (MQ=255)             gcgcTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCa                                                                                                                 >  2:159149/1‑139 (MQ=255)                         cATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTc                                                                                                     <  1:12992/139‑1 (MQ=255)                           tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTc                                                                                                     >  2:181537/1‑137 (MQ=255)                           tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTc                                                                                                     <  1:181537/137‑1 (MQ=255)                            cACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAg                                                                                                  <  1:945472/139‑1 (MQ=255)                             aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGc                                                                                                 >  1:668973/1‑139 (MQ=255)                                    tgaCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAc                                                                                          >  1:158016/1‑139 (MQ=255)                                     gaCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAcc                                                                                         <  2:62131/139‑1 (MQ=255)                                           cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             <  2:299113/97‑1 (MQ=255)                                           cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             >  1:299113/1‑97 (MQ=255)                                           cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAc                                                                                          <  1:896036/132‑1 (MQ=255)                                           cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAc                                                                                          >  2:896036/1‑132 (MQ=255)                                           cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                                                   >  1:91019/1‑139 (MQ=255)                                                tGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCa                                                                                                                        <  2:777812/97‑1 (MQ=255)                                                tGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCa                                                                                                                        >  1:777812/1‑97 (MQ=255)                                                tGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAccaccttcgataa                                                                              >  2:1052582/1‑128 (MQ=255)                                                tGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAtt                                                                              >  2:943270/1‑139 (MQ=255)                                                  cATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGt                                                                            >  2:856417/1‑139 (MQ=255)                                                     atCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGTCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCa                                                                                                                 <  2:1110771/99‑1 (MQ=255)                                                     atCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGTCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCa                                                                                                                 >  1:1110771/1‑99 (MQ=255)                                                        gTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATgg                                                                      >  2:622701/1‑139 (MQ=255)                                                         tCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGc                                                                                                                         <  1:103910/87‑1 (MQ=255)                                                         tCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGc                                                                                                                         >  2:103910/1‑87 (MQ=255)                                                          cAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGc                                                                    >  2:259267/1‑139 (MQ=255)                                                            aTGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAc                                                                  >  1:190470/1‑139 (MQ=255)                                                             tGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAcc                                                                 >  1:622072/1‑139 (MQ=255)                                                             tGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAcc                                                                 >  2:890144/1‑139 (MQ=255)                                                               cgcTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGa                                                               >  1:88052/1‑139 (MQ=255)                                                                    tCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCa                                                          <  1:159149/139‑1 (MQ=255)                                                                     ccGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACTGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt                                                         <  2:1046971/139‑1 (MQ=255)                                                                                aaCGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATg                                              <  1:475001/139‑1 (MQ=255)                                                                                aaCGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATg                                              <  2:190470/139‑1 (MQ=255)                                                                                  tgAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       >  1:692802/2‑64 (MQ=255)                                                                                  cGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  2:6014/64‑1 (MQ=255)                                                                                  cGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  2:692802/64‑1 (MQ=255)                                                                                  cGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       >  1:6014/1‑64 (MQ=255)                                                                                  cGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCg                                            >  1:111729/1‑139 (MQ=255)                                                                                          cTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac                                    <  1:895362/139‑1 (MQ=255)                                                                                          cTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac                                    <  1:622701/139‑1 (MQ=255)                                                                                             tCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATaaa                                                                                <  1:395189/92‑1 (MQ=255)                                                                                             tCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATaaa                                                                                >  2:395189/1‑92 (MQ=255)                                                                                              ccTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAaccacc                                >  2:262683/1‑139 (MQ=255)                                                                                                 gATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCttt                             >  2:492415/1‑139 (MQ=255)                                                                                                  aTGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTa                            >  1:237669/1‑139 (MQ=255)                                                                                                    gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAAc                          <  1:274136/139‑1 (MQ=255)                                                                                                    gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAAc                          <  2:91019/139‑1 (MQ=255)                                                                                                    gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAAc                          <  2:936007/139‑1 (MQ=255)                                                                                                    gCCAGGCGGAAAGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAAc                          <  1:1008368/139‑1 (MQ=255)                                                                                                         gCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGc                                                                    >  1:369748/1‑92 (MQ=255)                                                                                                         gCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGc                                                                    <  2:369748/92‑1 (MQ=255)                                                                                                         gCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCggg                                          >  1:1000849/1‑118 (MQ=255)                                                                                                         gCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCggg                                          <  2:1000849/118‑1 (MQ=255)                                                                                                         gCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATCGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGt                     >  1:805399/1‑139 (MQ=255)                                                                                                                gTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATAcc              <  2:622072/139‑1 (MQ=255)                                                                                                                    cGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGAc                                                              >  2:659870/1‑87 (MQ=255)                                                                                                                    cGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGAc                                                              <  1:659870/87‑1 (MQ=255)                                                                                                                            aTCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATg                                              <  1:735880/95‑1 (MQ=255)                                                                                                                            aTCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCa  <  2:158016/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        TCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCA  >  NC_000913/2829367‑2829628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑