Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,834,077	G→A	A56T (GCC→ACC)	norW →	NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,834,077	0	G	A	100.0%	139.0 / NA	41	A56T (GCC→ACC)	norW	NADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/17);  total (24/17)

TTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAA  >  NC_000913/2833973‑2834208                                                                                                         |                                                                                                                                    ttCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAAtt                                                                                                   >  1:1002407/1‑139 (MQ=255)       aaCAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGa                                                                                             <  1:358363/139‑1 (MQ=255)                    tATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTg                                                                                <  2:218659/139‑1 (MQ=255)                      ttCCATTAACCCCGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGc                                                                                                                    <  1:1110951/101‑1 (MQ=255)                      ttCCATTAACCCCGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGc                                                                                                                    >  2:1110951/1‑101 (MQ=255)                           ttAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         >  2:878079/1‑139 (MQ=255)                           ttAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         >  1:303729/1‑139 (MQ=255)                              aCCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTc                                                                      >  2:726888/1‑139 (MQ=255)                               cccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTcc                                                                     <  1:214956/139‑1 (MQ=255)                                ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca                                                                    >  1:176252/1‑139 (MQ=255)                                ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca                                                                    >  1:19714/1‑139 (MQ=255)                                ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGAAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCca                                                                    >  1:1061549/1‑139 (MQ=255)                                   gATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg                                                                                            <  2:634944/112‑1 (MQ=255)                                   gATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg                                                                                            >  1:634944/1‑112 (MQ=255)                                            cgACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTg                                                        <  1:878079/139‑1 (MQ=255)                                             gACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGa                                                       <  2:206764/139‑1 (MQ=255)                                              aCAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGcc                                                                                               >  1:1102214/1‑98 (MQ=255)                                              aCAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCa                                                                              <  2:365736/115‑1 (MQ=255)                                              aCAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCa                                                                              >  1:365736/1‑115 (MQ=255)                                              aCAGCATGGATGAGTAAAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGcc                                                                                               <  2:1102214/98‑1 (MQ=255)                                                      gATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCg                                              <  2:1002407/139‑1 (MQ=255)                                                          aGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGc                                          >  1:264869/1‑139 (MQ=255)                                                            tACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         <  2:438207/106‑1 (MQ=255)                                                            tACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGt                                                                         >  1:438207/1‑106 (MQ=255)                                                            tACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCg                                        >  1:681865/1‑139 (MQ=255)                                                               aacaaACCTGATCTCAGCCAAGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAg                                     >  1:898693/1‑139 (MQ=255)                                                                 caaACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGcc                                   >  1:1082118/1‑139 (MQ=255)                                                                      cTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCgtgt                              >  1:521675/1‑139 (MQ=255)                                                                         aCCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTgtggt                           >  1:899244/1‑139 (MQ=255)                                                                           cTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGa                         >  2:432715/1‑139 (MQ=255)                                                                                ccATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGc                    <  2:959739/139‑1 (MQ=255)                                                                                ccATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGc                    <  2:521675/139‑1 (MQ=255)                                                                                 cATGTTATCAGTCAGGGTCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTTGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGcg                   <  2:681865/139‑2 (MQ=255)                                                                                              agGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTg      >  1:499524/1‑139 (MQ=255)                                                                                               ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGcc                   <  1:914418/125‑1 (MQ=255)                                                                                               ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGcc                   >  2:914418/1‑125 (MQ=255)                                                                                               ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTgg     >  1:270637/1‑139 (MQ=255)                                                                                               ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTgg     >  1:820751/1‑139 (MQ=255)                                                                                                gggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGc    >  1:624117/1‑139 (MQ=255)                                                                                                  gCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCaa  <  2:499524/139‑1 (MQ=255)                                                                                                  gCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCaa  <  2:270637/139‑1 (MQ=255)                                                                                                         |                                                                                                                                    TTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAA  >  NC_000913/2833973‑2834208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑