Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,066,288 T→C T296A (ACG→GCG)  ygfI ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,066,2880TC97.9% 356.9 / ‑0.4 96coding (886/897 nt)ygfIputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (45/49);  minor base . (1/1);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

CCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCC  >  NC_000913/3066155‑3066412
                                                                                                                                     |                                                                                                                            
ccTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGt                                                                                                                         >  1:388975/1‑139 (MQ=255)
   aTGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCAc                                                                                                                      >  1:943544/1‑139 (MQ=255)
    tGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACg                                                                                                                     >  2:493790/1‑139 (MQ=255)
        tCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCt                                                                                                                 >  1:591058/1‑139 (MQ=255)
          gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTc                                                                                                               >  2:333012/1‑139 (MQ=255)
          gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTc                                                                                                               >  1:53922/1‑139 (MQ=255)
          gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTc                                                                                                               >  2:686667/1‑139 (MQ=255)
          gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATATAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTc                                                                                                               >  1:1091111/1‑139 (MQ=255)
           ggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCa                                                                                                              >  2:825887/1‑139 (MQ=255)
              ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACg                                                                                                                     <  1:176858/129‑1 (MQ=255)
              ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACg                                                                                                                     >  2:176858/1‑129 (MQ=255)
              ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCa                                                                                                           <  2:713879/139‑1 (MQ=255)
               tATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCaa                                                                                                          <  2:628743/139‑1 (MQ=255)
                aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCaa                                                                                                          >  1:735668/1‑138 (MQ=255)
                aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCaa                                                                                                          <  2:735668/138‑1 (MQ=255)
                          cTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACTTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAg                                                                                                <  2:963310/138‑1 (MQ=255)
                          cTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAg                                                                                                >  1:963310/1‑138 (MQ=255)
                                 gAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  2:37690/139‑1 (MQ=255)
                                 gAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  1:256251/139‑1 (MQ=255)
                                 gAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  1:333012/139‑1 (MQ=255)
                                   aaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGTTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCg                                                                                      >  2:995142/1‑139 (MQ=255)
                                    aaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCgg                                                                                     <  2:1043205/139‑1 (MQ=255)
                                       gcgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtat                                                                                  >  2:976809/1‑139 (MQ=255)
                                        cgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        >  1:799480/1‑132 (MQ=255)
                                        cgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  2:799480/132‑1 (MQ=255)
                                            tGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                                             <  2:1091111/139‑1 (MQ=255)
                                            tGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                                             <  1:825887/139‑1 (MQ=255)
                                            tGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                                             >  2:314405/1‑139 (MQ=255)
                                                ggACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACgatgat                                                                         <  1:893427/139‑1 (MQ=255)
                                                      atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGa                                                                   <  1:157064/139‑1 (MQ=255)
                                                      atTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGa                                                                   <  1:686667/139‑1 (MQ=255)
                                                        tCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAtt                                                                 >  1:575285/1‑139 (MQ=255)
                                                         caccacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTg                                                                >  2:897285/1‑139 (MQ=255)
                                                            cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                             <  2:575285/139‑1 (MQ=255)
                                                            cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                             <  1:976809/139‑1 (MQ=255)
                                                              cGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa                                                           >  2:110210/1‑139 (MQ=255)
                                                              cGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa                                                          >  2:414408/1‑139 (MQ=255)
                                                                 tCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                        <  1:249123/139‑1 (MQ=255)
                                                                   ttAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                      <  1:314405/139‑1 (MQ=255)
                                                                   ttAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCCTAGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                      >  1:751069/1‑139 (MQ=255)
                                                                    tAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                        <  2:547787/136‑1 (MQ=255)
                                                                    tAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                                        >  1:547787/1‑136 (MQ=255)
                                                                    tAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATAtt                                                    <  1:414408/139‑1 (MQ=255)
                                                                     aGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATAtt                                                    <  2:592906/139‑1 (MQ=255)
                                                                     aGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATAtt                                                    <  2:890801/139‑1 (MQ=255)
                                                                      gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGTTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  1:591770/102‑1 (MQ=255)
                                                                      gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGTTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        >  2:591770/1‑102 (MQ=255)
                                                                      gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                   <  2:388975/139‑1 (MQ=255)
                                                                      gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                   >  1:951671/1‑139 (MQ=255)
                                                                      gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                                                   <  1:493790/139‑1 (MQ=255)
                                                                       ccACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTcc                                                  <  1:897285/139‑1 (MQ=255)
                                                                          cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTc                                                                      <  2:633900/116‑1 (MQ=255)
                                                                          cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTc                                                                      >  1:633900/1‑116 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGTAATATGCTGAAGCATCGTgg                                                                                        >  2:428337/1‑97 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGTAATATGCTGAAGCATCGTgg                                                                                        <  1:428337/97‑1 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtata                                                                                 >  2:638517/1‑104 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtata                                                                                 <  1:638517/104‑1 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGATTAAAGATATTCCTGCa                                              >  2:753264/1‑139 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTcc                                                  >  2:771853/1‑135 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTcc                                                  <  1:771853/135‑1 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                              >  2:100035/1‑139 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                              >  1:166933/1‑139 (MQ=255)
                                                                           gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                                              >  1:221385/1‑139 (MQ=255)
                                                                            tCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCat                                             <  2:221385/139‑1 (MQ=255)
                                                                            tCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCat                                             <  1:753264/139‑1 (MQ=255)
                                                                             cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGt                                                                                          >  1:597652/1‑93 (MQ=255)
                                                                             cTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGt                                                                                          <  2:597652/93‑1 (MQ=255)
                                                                                 gACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACa                                        <  2:380736/139‑1 (MQ=255)
                                                                                       cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  1:131220/85‑1 (MQ=255)
                                                                                       cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        >  2:131220/1‑85 (MQ=255)
                                                                                           gTAATTTAATTGCAACCGGTTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCtgtg                              <  1:995142/139‑1 (MQ=255)
                                                                                            taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCaa                                                                                                          <  2:264088/62‑1 (MQ=255)
                                                                                            taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCaa                                                                                                          >  1:264088/1‑62 (MQ=255)
                                                                                            taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  2:747124/80‑1 (MQ=255)
                                                                                            taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        >  1:747124/1‑80 (MQ=255)
                                                                                            taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTg                                                                                         >  1:924202/1‑79 (MQ=255)
                                                                                            taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTg                                                                                         <  2:924202/79‑1 (MQ=255)
                                                                                             aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  1:945591/79‑1 (MQ=255)
                                                                                             aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        >  2:945591/1‑79 (MQ=255)
                                                                                              atttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg                           <  1:110210/139‑1 (MQ=255)
                                                                                                  aattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtata                                                                                 >  2:828817/1‑81 (MQ=255)
                                                                                                  aattGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtata                                                                                 <  1:828817/81‑1 (MQ=255)
                                                                                                   attGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt                      <  2:951671/139‑1 (MQ=255)
                                                                                                    ttGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATc                     >  2:355818/1‑139 (MQ=255)
                                                                                                         aCCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACg                                                                                                                     <  2:142936/38‑1 (MQ=255)
                                                                                                         aCCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACg                                                                                                                     >  1:142936/1‑38 (MQ=255)
                                                                                                            ggCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                      <  1:937906/98‑1 (MQ=255)
                                                                                                            ggCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                                      >  2:937906/1‑98 (MQ=255)
                                                                                                                cGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAg         <  2:751069/139‑1 (MQ=255)
                                                                                                                       gggTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGcc  >  1:630735/1‑139 (MQ=255)
                                                                                                                        ggTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCt                                                 <  2:715481/91‑1 (MQ=255)
                                                                                                                        ggTAAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCt                                                 >  1:715481/1‑91 (MQ=255)
                                                                                                                             aTGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        >  1:38026/1‑47 (MQ=255)
                                                                                                                             aTGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                                        <  2:38026/47‑1 (MQ=255)
                                                                                                                             aTGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                             <  1:514355/74‑1 (MQ=255)
                                                                                                                             aTGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                                             >  2:514355/1‑74 (MQ=255)
                                                                                                                                     |                                                                                                                            
CCTATGTATCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCC  >  NC_000913/3066155‑3066412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: