| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 467,458 | C→T | P16L (CCC→CTC) | cof → | thiamine pyrimidine pyrophosphate hydrolase, HMP‑PP phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 467,458 | 0 | C | T | 98.3% | 202.8 / NA | 60 | P16L (CCC→CTC) | cof | thiamine pyrimidine pyrophosphate hydrolase, HMP‑PP phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (33/26); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG > NC_000913/467323‑467591 | aaCAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGa > 2:28468/1‑139 (MQ=255)aaCAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGa > 1:853470/1‑139 (MQ=255) aCAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGAc < 1:1096824/139‑1 (MQ=255) cTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTga > 1:315490/1‑139 (MQ=255) cTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTg > 1:1094003/1‑138 (MQ=255) cTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTg < 2:1094003/138‑1 (MQ=255) cTATTCACAATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCAGCATTTAGGTga > 2:720587/1‑139 (MQ=255) aCTATTACTTTATTATAAAGAAGGTGATACTCAATCACTATTAACCGTGTCAAAGGGTGAAAAAAGAAATGCCTCTTCTGGCAGAATTTGATATGGATGGCATTTTATTGATGCCCGACAATCTTTTAGGTGA‑AAAAcc < 1:475582/139‑1 (MQ=255) cTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAAcc < 1:28468/139‑1 (MQ=255) ccGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACt > 2:511825/1‑139 (MQ=255) gTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACttt > 1:985211/1‑139 (MQ=255) aGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACt > 1:946750/1‑139 (MQ=255) gTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACca > 2:15132/1‑98 (MQ=255) gTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACca < 1:15132/98‑1 (MQ=255) gTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGc > 1:475130/1‑139 (MQ=255) gTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGc > 2:614382/1‑139 (MQ=255) gTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGc > 2:462940/1‑139 (MQ=255) aTACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTg < 1:18185/139‑1 (MQ=255) aTACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTg > 1:85840/1‑139 (MQ=255) tACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGa > 2:181596/1‑139 (MQ=255) aCTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGaa > 1:841125/1‑139 (MQ=255) aCTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGaa > 1:68592/1‑139 (MQ=255) aCTCAATCACCATTAACCGGGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGaa < 1:720587/139‑1 (MQ=255) tCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAcg < 2:985211/139‑1 (MQ=255) aTCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGgc < 1:362479/121‑1 (MQ=255) aTCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGgc > 2:362479/1‑121 (MQ=255) aTCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGa > 1:759882/1‑139 (MQ=255) cACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACa < 1:285570/139‑1 (MQ=255) aaCCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCt < 1:418850/139‑1 (MQ=255) aaCCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCt > 2:418850/1‑139 (MQ=255) tgtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGaaaa < 1:810050/91‑1 (MQ=255) tgtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGACATGGATGGCCCTTTATTGATGCTCGACCATCATTTAGGTGAGaaaa > 2:810050/1‑91 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGAATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACttt > 2:705731/1‑139 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAAcc > 2:154766/1‑83 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAAcc < 1:154766/83‑1 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACggg > 2:747446/1‑139 (MQ=255) agaaagaaATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCg > 2:759726/1‑139 (MQ=255) aaTGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTTAACGCGACATTACCCTCACTTTTGCCACgggg < 1:659140/130‑1 (MQ=255) aaTGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGATGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACgggg > 2:659140/1‑130 (MQ=255) tCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGgag < 2:759882/139‑1 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGAc < 1:1025690/43‑1 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGAc > 2:1025690/1‑43 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg < 1:747446/139‑1 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg < 1:462940/139‑1 (MQ=255) aTTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATAtt < 2:68592/139‑1 (MQ=255) tttGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATtc > 1:814824/1‑139 (MQ=255) atatGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCgg < 2:841125/139‑1 (MQ=255) atggatggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCgggg < 1:791530/139‑1 (MQ=255) gatggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCt > 2:536327/1‑86 (MQ=255) gatggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCt < 1:536327/86‑1 (MQ=255) cACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCctct > 1:665373/1‑44 (MQ=255) cACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCctct < 2:665373/44‑1 (MQ=255) aCTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGc > 1:1038709/1‑139 (MQ=255) tATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGa > 2:143365/1‑139 (MQ=255) tATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGa < 1:511825/139‑1 (MQ=255) aTTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGAt > 1:416644/1‑139 (MQ=255) gATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCg < 2:1038709/139‑1 (MQ=255) gATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCg < 2:946750/139‑1 (MQ=255) aTGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACa > 1:914595/1‑64 (MQ=255) aTGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACa < 2:914595/64‑1 (MQ=255) | AACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG > NC_000913/467323‑467591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |