Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,638,618	T→C	V326A (GTG→GCG)	pitA →	phosphate transporter, low‑affinity, tellurite importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,638,618	0	T	C	100.0%	144.9 / NA	38	V326A (GTG→GCG)	pitA	phosphate transporter, low‑affinity, tellurite importer
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/20);  total (18/20)

TCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGAGTGCTGACGATCAGCGCCTGTTGAAGA  >  NC_000913/3638483‑3638749                                                                                                                                        |                                                                                                                                    tCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGTGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGa                                                                                                                                  <  1:869874/139‑1 (MQ=255)      cAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCt                                                                                                                             <  1:168908/139‑1 (MQ=255)          cTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGa                                                                                                                         >  1:891948/1‑139 (MQ=255)          cTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGa                                                                                                                         >  2:215935/1‑139 (MQ=255)           tACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGAc                                                                                                                        <  2:458052/139‑1 (MQ=255)                   cTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTc                                                                                                                <  1:459668/139‑1 (MQ=255)                   cTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTc                                                                                                                >  1:575104/1‑139 (MQ=255)                           ttAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATc                                                                                                        >  1:67802/1‑139 (MQ=255)                                   ggCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGc                                                                                                >  1:346995/1‑139 (MQ=255)                                           aaGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATgcg                                                                                        <  1:485524/139‑1 (MQ=255)                                           aaGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATgcg                                                                                        <  2:834604/139‑1 (MQ=255)                                                ggCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCa                                                                                   <  1:801194/139‑1 (MQ=255)                                                       cgcaacCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCt                                                                            >  2:770285/1‑139 (MQ=255)                                                                      tCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATgcg                                                                                        >  1:228450/1‑112 (MQ=255)                                                                      tCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATgcg                                                                                        <  2:228450/112‑1 (MQ=255)                                                                      tCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGacac                                                             <  2:346995/139‑1 (MQ=255)                                                                       ccACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACt                                                            <  1:41392/139‑1 (MQ=255)                                                                             ccATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGAc                                                      <  1:711768/139‑1 (MQ=255)                                                                              cATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACa                                                     <  2:891948/139‑1 (MQ=255)                                                                              cATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACa                                                     <  2:125018/139‑1 (MQ=255)                                                                               aTCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACaa                                                    <  1:770285/139‑1 (MQ=255)                                                                                tCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACaaa                                                   >  2:484072/1‑139 (MQ=255)                                                                                       aaTACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGa                                                                >  2:684413/1‑119 (MQ=255)                                                                                       aaTACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGa                                                                <  1:684413/119‑1 (MQ=255)                                                                                                 aCGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGa                                                                                                                                  >  1:491513/1‑43 (MQ=255)                                                                                                 aCGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGa                                                                                                                                  <  2:491513/43‑1 (MQ=255)                                                                                                 aCGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTgg                                  >  1:578414/1‑139 (MQ=255)                                                                                                  cgcgCTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGTGCCGCATTATGCTGTGCGTTTCTGACACTATCGCCAAAGTGGTGAAGATGCCTGGc                                 >  2:804680/1‑139 (MQ=255)                                                                                                    cgcTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGt                               <  2:578414/139‑1 (MQ=255)                                                                                                     gcTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATgcg                                                                                        >  1:234350/1‑81 (MQ=255)                                                                                                     gcTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATgcg                                                                                        <  2:234350/81‑1 (MQ=255)                                                                                                      cTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGa                             >  1:691703/1‑139 (MQ=255)                                                                                                      cTCAACCGCCTGAAAGGTATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGa                             >  1:992450/1‑139 (MQ=255)                                                                                                                       tATGTTGACCACCGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGAGTGCTGACGATCAgcgc            <  2:67802/139‑1 (MQ=255)                                                                                                                                caccGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGAGTGCTGACGATCAGCGCCTGTTgaag   >  2:733556/1‑139 (MQ=255)                                                                                                                                 accGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGCGAGTGCTGACGATCAGCGCCTGTTgaaga  >  2:203418/1‑139 (MQ=255)                                                                                                                                   cGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGAGTGCTGa                      <  2:76984/117‑1 (MQ=255)                                                                                                                                   cGATGCGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGAGTGCTGa                      >  1:76984/1‑117 (MQ=255)                                                                                                                                        |                                                                                                                                    TCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAGCTACGACAAGCTGTCGCTTGATCAACGTAGCCAGATGCGCCGCATTATGCTGTGCGTTTCTGACACTATCGACAAAGTGGTGAAGATGCCTGGCGTGAGTGCTGACGATCAGCGCCTGTTGAAGA  >  NC_000913/3638483‑3638749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑