| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,790,538 | A→G | S270P (TCT→CCT) | tdh ← | L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,790,538 | 0 | A | G | 96.1% | 174.6 / ‑1.9 | 51 | S270P (TCT→CCT) | tdh | L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (27/22); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01 | |||||||||||
TGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACT > NC_000913/3790405‑3790669 | tGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCatat > 1:293036/1‑139 (MQ=255) aTGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGAcg < 2:1028488/139‑1 (MQ=255) aTGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcg < 1:229647/139‑1 (MQ=255) aTGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcg > 2:229647/1‑139 (MQ=255) tcgCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGa < 2:734339/137‑1 (MQ=255) ggCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGaa > 1:1016545/1‑139 (MQ=255) ggCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGaa > 2:475242/1‑139 (MQ=255) aGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGt < 2:958378/139‑1 (MQ=255) ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGAtt > 2:481510/1‑139 (MQ=255) gccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa < 1:906461/139‑1 (MQ=255) ccAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCTTATCAGACGGCGGAATACCGAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc > 1:265211/1‑139 (MQ=255) ccAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGa > 2:973242/1‑87 (MQ=255) ccAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGa < 1:973242/87‑1 (MQ=255) gTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATgg > 1:618858/1‑139 (MQ=255) gTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATgg > 2:863670/1‑139 (MQ=255) tttCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGt > 2:28356/1‑139 (MQ=255) cATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAAc < 1:475242/139‑1 (MQ=255) ctcACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCt > 1:622435/1‑139 (MQ=255) ctcACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCt > 2:728363/1‑139 (MQ=255) cGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcg < 2:618858/139‑1 (MQ=255) gACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcgg < 2:622435/139‑1 (MQ=255) tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCacac > 1:268992/1‑139 (MQ=255) tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAc > 1:1023113/1‑139 (MQ=255) tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAc > 1:617341/1‑139 (MQ=255) tACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa < 1:863670/139‑1 (MQ=255) tACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa < 2:265211/139‑1 (MQ=255) ttAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAttt > 2:449748/1‑139 (MQ=255) aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGTAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGAc < 1:792948/133‑1 (MQ=255) aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGAc > 2:792948/1‑133 (MQ=255) aTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCGATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCa > 1:1073769/1‑139 (MQ=255) aCAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGAcc < 1:479646/139‑1 (MQ=255) cAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc > 1:84936/1‑74 (MQ=255) cAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc < 2:84936/74‑1 (MQ=255) cAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCTCCGTGATTCTTGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGAc < 1:685158/127‑1 (MQ=255) cAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGAc > 2:685158/1‑127 (MQ=255) cAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg > 1:447626/1‑139 (MQ=255) cTTTAAAGATCACTTTGGTCCAGTCGATATGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCTTGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATc > 1:538650/1‑139 (MQ=255) tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcg > 1:467993/1‑108 (MQ=255) tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcg < 2:467993/108‑1 (MQ=255) tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCaaa > 2:372592/1‑139 (MQ=255) tCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgtgt > 2:260846/1‑78 (MQ=255) tCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgtgt < 1:260846/78‑1 (MQ=255) cACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc < 2:717695/82‑1 (MQ=255) cACTTTGGTCCAGTCGATAGGCATATCAAACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc > 1:717695/1‑82 (MQ=255) aCTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCgg < 1:28356/139‑1 (MQ=255) aCTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCgg < 1:481510/139‑1 (MQ=255) tttGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTc < 1:728363/139‑1 (MQ=255) tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa < 2:512961/98‑1 (MQ=255) tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa > 1:512961/1‑98 (MQ=255) tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACt > 2:1091549/1‑139 (MQ=255) tAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGccgccg < 1:1074983/47‑1 (MQ=255) tAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGccgccg > 2:1074983/1‑47 (MQ=255) | TGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACT > NC_000913/3790405‑3790669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |