Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,121	A→G	N269S (AAC→AGC)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,121	0	A	G	100.0%	169.2 / NA	45	N269S (AAC→AGC)	kup	potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/16);  total (29/16)

TCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCA  >  NC_000913/3931985‑3932256                                                                                                                                         |                                                                                                                                        tCGATTACGGGGGACGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGcc                                                                                                                                       >  2:595026/1‑139 (MQ=255)      tACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGaa                                                                                                                                  >  2:688463/1‑139 (MQ=255)                   gcgcTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAAcc                                                                                                                     <  2:51839/139‑1 (MQ=255)                                   tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                                                                     >  1:111110/1‑139 (MQ=255)                                   tGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                                                                     >  2:544435/1‑139 (MQ=255)                                     gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCgg                                                                                                                                    <  2:995271/106‑1 (MQ=255)                                     gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCgg                                                                                                                                    >  1:995271/1‑106 (MQ=255)                                     gggCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAc                                                                                                   >  2:838007/1‑139 (MQ=255)                                     gggCACCTTGGTAACTTCCCTATTCACCTGGCGTGGTTCACCGTCGCATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAc                                                                                                   >  1:1029184/1‑139 (MQ=255)                                                  aGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCt                                                                                      <  1:544435/139‑1 (MQ=255)                                                       ccTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                                 >  2:39376/1‑139 (MQ=255)                                                       ccTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATcc                                                                                 <  2:371354/139‑1 (MQ=255)                                                         tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGa                                                                                                                        >  1:177152/1‑98 (MQ=255)                                                         tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGa                                                                                                                        <  2:177152/98‑1 (MQ=255)                                                         tATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCg                                                                               >  2:1060248/1‑139 (MQ=255)                                                                        gTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgcc                                                                >  2:394917/1‑139 (MQ=255)                                                                         ttCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgccg                                                               >  1:469984/1‑139 (MQ=255)                                                                             ccGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACt                                                           >  1:390784/1‑139 (MQ=255)                                                                                tcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                           <  1:710774/88‑1 (MQ=255)                                                                                tcgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                                           >  2:710774/1‑88 (MQ=255)                                                                                 cgtATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCg                                                       >  2:163019/1‑139 (MQ=255)                                                                                   tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGa                                                                                               >  1:445441/1‑97 (MQ=255)                                                                                   tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGa                                                                                               <  2:445441/97‑1 (MQ=255)                                                                                   tATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGAc                                                     >  1:424749/1‑139 (MQ=255)                                                                                            ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGc                                                                                                                                <  2:841664/55‑1 (MQ=255)                                                                                            ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGc                                                                                                                                >  1:841664/1‑55 (MQ=255)                                                                                            ccttGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGc                                            >  1:1077898/1‑139 (MQ=255)                                                                                                  cccTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCa                                      <  1:595026/139‑1 (MQ=255)                                                                                                        aTTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                                <  1:688463/139‑1 (MQ=255)                                                                                                        aTTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGt                                <  1:838007/139‑1 (MQ=255)                                                                                                             ttCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTAtct                           >  1:277699/1‑139 (MQ=255)                                                                                                              tCGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTAtctc                          >  2:441391/1‑139 (MQ=255)                                                                                                               cGGTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACCGGCGACGGTAATTGCCTCGCAGGCGGTTATctct                         >  2:399181/1‑139 (MQ=255)                                                                                                                ggTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTg                        >  2:642379/1‑139 (MQ=255)                                                                                                                 gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCg                                                                                                                   <  2:13674/47‑1 (MQ=255)                                                                                                                 gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCg                                                                                                                   >  1:13674/1‑47 (MQ=255)                                                                                                                 gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTgg                       >  1:318556/1‑139 (MQ=255)                                                                                                                 gTCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTgg                       >  2:707468/1‑139 (MQ=255)                                                                                                                  tCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                      <  1:395490/139‑1 (MQ=255)                                                                                                                  tCAGGGAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGc                      <  1:83448/139‑1 (MQ=255)                                                                                                                     gggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtc                   <  2:1077898/139‑1 (MQ=255)                                                                                                                      ggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtct                  >  1:744386/1‑139 (MQ=255)                                                                                                                      ggAGCGCTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGtct                  >  2:390478/1‑139 (MQ=255)                                                                                                                          cgcTGTTGTTAAAGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTtctc              <  1:390478/139‑1 (MQ=255)                                                                                                                                    aaaGAGCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGt    >  1:284432/1‑139 (MQ=255)                                                                                                                                      agagCCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCa  <  2:113242/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCA  >  NC_000913/3931985‑3932256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑