Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,238	C→T	A665V (GCG→GTG)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,238	0	C	T	100.0%	142.8 / NA	41	A665V (GCG→GTG)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/22);  total (19/22)

CTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGT  >  NC_000913/4183104‑4183373                                                                                                                                       |                                                                                                                                        cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGGTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTcc                                                                                                                                     <  2:613256/139‑1 (MQ=255)        ttGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATcc                                                                                                                              <  1:735022/139‑1 (MQ=255)            aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGtt                                                                                                                          >  2:396210/1‑139 (MQ=255)                    ggCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAac                                                                                                                  >  1:498941/1‑139 (MQ=255)                       cACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACg                                                                                                               <  1:183479/139‑1 (MQ=255)                         cTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGAt                                                                                                             >  2:420630/1‑139 (MQ=255)                            cGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGAc                                                                                                          >  2:618606/1‑139 (MQ=255)                                  agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAc                                                                                                    <  1:588700/139‑1 (MQ=255)                                     ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGt                                                                                                 <  1:364395/139‑1 (MQ=255)                                     ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGt                                                                                                 <  2:171274/139‑1 (MQ=255)                                     ccTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGt                                                                                                 <  2:1107295/139‑1 (MQ=255)                                              ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                                                     >  1:577021/1‑126 (MQ=255)                                              ttGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCaa                                                                                                     <  2:577021/126‑1 (MQ=255)                                                    tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCg                                                                                  >  1:16322/1‑139 (MQ=255)                                                           ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGc                                                                           >  1:325993/1‑139 (MQ=255)                                                                     gCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGc                                                                 <  1:317947/139‑1 (MQ=255)                                                                         gTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGtt                                                             >  1:1058672/1‑139 (MQ=255)                                                                          ttCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTc                                                            >  1:873313/1‑139 (MQ=255)                                                                                      cAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                                                <  2:325993/139‑1 (MQ=255)                                                                                              cTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                                        <  1:618606/139‑1 (MQ=255)                                                                                                      aCGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                >  2:546859/1‑139 (MQ=255)                                                                                                      aCGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                <  1:791991/139‑1 (MQ=255)                                                                                                      aCGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                <  1:1025417/139‑1 (MQ=255)                                                                                                       cGTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggtt                               <  1:887733/139‑1 (MQ=255)                                                                                                        gTATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg                              >  2:415176/1‑139 (MQ=255)                                                                                                         tATCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                             <  1:900485/139‑1 (MQ=255)                                                                                                           tCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                           >  2:830501/1‑139 (MQ=255)                                                                                                           tCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                           <  1:420630/139‑1 (MQ=255)                                                                                                           tCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                           <  1:396210/139‑1 (MQ=255)                                                                                                           tCCACCCAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTa                           <  2:364576/139‑1 (MQ=255)                                                                                                                ccAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGt                      >  1:391773/1‑139 (MQ=255)                                                                                                                ccAGCAGGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGt                      >  2:522202/1‑139 (MQ=255)                                                                                                                  agcagGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGc                                                                                                        <  2:916199/55‑1 (MQ=255)                                                                                                                  agcagGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGc                                                                                                        >  1:916199/1‑55 (MQ=255)                                                                                                                    cagGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAcc                                                                                                   >  1:836300/1‑58 (MQ=255)                                                                                                                    cagGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAcc                                                                                                   <  2:836300/58‑1 (MQ=255)                                                                                                                    cagGTGGTATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATgg                  >  2:499057/1‑139 (MQ=255)                                                                                                                         ggtATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGt             <  2:1030173/139‑1 (MQ=255)                                                                                                                          gtATCCGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTg            >  2:237439/1‑139 (MQ=255)                                                                                                                              ccGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                >  2:118594/1‑115 (MQ=255)                                                                                                                              ccGTCGGTGTGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                                <  1:118594/115‑1 (MQ=255)                                                                                                                                    gtgtgtCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGt  >  2:539278/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        CTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGT  >  NC_000913/4183104‑4183373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑