Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,935	A→G	L69L (TTG→CTG)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,935	0	A	G	100.0%	264.3 / NA	69	L69L (TTG→CTG)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/38);  total (31/38)

TCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAG  >  NC_000913/4490803‑4491059                                                                                                                                     |                                                                                                                             tCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                        >  2:1075298/1‑139 (MQ=255)   aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                      <  2:38564/139‑1 (MQ=255)   aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                      <  1:155973/139‑1 (MQ=255)   aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                      <  2:61908/139‑1 (MQ=255)     cGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAgg                                                                                                                    >  2:606340/1‑139 (MQ=255)       aCTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGt                                                                                                                  >  2:100428/1‑139 (MQ=255)        ctctTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGta                                                                                                                 <  2:746896/139‑1 (MQ=255)                cGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTtg                                                                                                         >  2:945772/1‑139 (MQ=255)                          aTCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaac                                                                                               <  1:95526/139‑1 (MQ=255)                            cGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaacaa                                                                                             <  1:100428/139‑1 (MQ=255)                            cGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaacaa                                                                                             <  2:613221/139‑1 (MQ=255)                                      attAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGa                                                                                   >  1:1096365/1‑139 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                        >  2:766108/1‑101 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                        <  1:766108/101‑1 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  2:321082/139‑1 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTATGAAAGGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  2:86517/139‑1 (MQ=255)                                        tAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAgc                                                                                 <  1:165917/139‑1 (MQ=255)                                         aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCg                                                                                                                       <  1:510811/100‑1 (MQ=255)                                         aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCg                                                                                                                       >  2:510811/1‑100 (MQ=255)                                         aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcg                                                                                >  1:1096473/1‑139 (MQ=255)                                             aTAAGGGTCTGATAATTATGCTCAGAGGTGACGGATGCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAt                                                                            <  2:807604/139‑1 (MQ=255)                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:998558/139‑1 (MQ=255)                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           >  1:943114/1‑139 (MQ=255)                                                aGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAg                                                                         <  2:609310/139‑1 (MQ=255)                                                   gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:336933/139‑1 (MQ=255)                                                   gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:566290/139‑1 (MQ=255)                                                   gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:625951/139‑1 (MQ=255)                                                   gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  2:943114/139‑1 (MQ=255)                                                   gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGCTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGTATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  1:672173/139‑1 (MQ=255)                                                     cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGata                                                                    >  1:822420/1‑139 (MQ=255)                                                     cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGata                                                                    <  1:238130/139‑1 (MQ=255)                                                      tGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGcc                                                                                       >  2:94764/1‑119 (MQ=255)                                                      tGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGcc                                                                                       <  1:94764/119‑1 (MQ=255)                                                            ttATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                        >  2:551521/1‑80 (MQ=255)                                                            ttATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                        <  1:551521/80‑1 (MQ=255)                                                             tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                            >  2:604641/1‑139 (MQ=255)                                                                  tCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTca                                                                                                 <  2:313411/97‑1 (MQ=255)                                                                  tCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTca                                                                                                 >  1:313411/1‑97 (MQ=255)                                                                    agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTcc                                                     >  1:1110428/1‑139 (MQ=255)                                                                     gagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCa                                                    <  1:945772/139‑1 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      >  2:346640/1‑120 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                      <  1:346640/120‑1 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                   >  1:908642/1‑139 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                   >  1:611703/1‑139 (MQ=255)                                                                         tGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAAt                                                >  2:972957/1‑139 (MQ=255)                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  <  2:1438/103‑1 (MQ=255)                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                  >  1:1438/1‑103 (MQ=255)                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAtc                                                           <  2:29030/126‑1 (MQ=255)                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAtc                                                           >  1:29030/1‑126 (MQ=255)                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAAttt                                              >  2:315754/1‑139 (MQ=255)                                                                                ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGAGCATTCAACAACATGCCGGGAg                                                                                  >  1:110711/1‑98 (MQ=255)                                                                                ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGAGCATTCAACAACATGCCGGGAg                                                                                  <  2:110711/98‑1 (MQ=255)                                                                                  cTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGAt                                       <  1:606340/139‑1 (MQ=255)                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGcc                              >  2:264974/1‑139 (MQ=255)                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGcc                              >  1:757947/1‑139 (MQ=255)                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATGTTGGCGATGCGCTCGcc                              >  2:683671/1‑139 (MQ=255)                                                                                                         aaGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGAGCTCGCCTGTTTCCTTTGCCa                >  2:653342/1‑139 (MQ=255)                                                                                                          agagTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTGGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCt                      >  2:1012086/1‑132 (MQ=255)                                                                                                          agagTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTGGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCt                      <  1:1012086/132‑1 (MQ=255)                                                                                                            agTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaacaa                                                                                             <  1:140504/59‑1 (MQ=255)                                                                                                            agTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaacaa                                                                                             >  2:140504/1‑59 (MQ=255)                                                                                                            agTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           <  1:70190/77‑1 (MQ=255)                                                                                                            agTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                           >  2:70190/1‑77 (MQ=255)                                                                                                                  ttttGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCg       <  2:896836/139‑1 (MQ=255)                                                                                                                      gAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                            <  1:810447/82‑1 (MQ=255)                                                                                                                      gAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                            >  2:810447/1‑82 (MQ=255)                                                                                                                      gAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCATTTGCCACCTTTTTCGGCGa   <  1:653342/139‑1 (MQ=255)                                                                                                                       aaaTGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAg  <  1:264974/139‑1 (MQ=255)                                                                                                                       aaaTGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAg  <  2:1110428/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                             TCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAG  >  NC_000913/4490803‑4491059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑