Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	585,317	C→T	W106* (TGG→TAG)	ompT ←	DLP12 prophage, outer membrane protease VII, outer membrane protein 3b

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	585,317	0	C	T	100.0%	123.6 / NA	36	W106* (TGG→TAG)	ompT	DLP12 prophage, outer membrane protease VII, outer membrane protein 3b
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

TCCCAGGCGGTAATTGGGTTCGTTGAGGAGCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCCAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCC  >  NC_000913/585187‑585443                                                                                                                                   |                                                                                                                               tCCCAGGCGGTAATTGGGTTCGTTGAGGAGCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGa                                                                                                                        >  1:703558/1‑139 (MQ=255)           tAATTGGGTTCGTTGAGGAGCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCatat                                                                                                              >  1:296239/1‑139 (MQ=255)                     cGTTGAGGAGCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCg                                                                                                    >  2:503464/1‑139 (MQ=255)                      gTTGAGGAGCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCgg                                                                                                   <  1:661753/139‑1 (MQ=255)                           ggagCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGcc                                                                                              <  2:296239/139‑1 (MQ=255)                               ccagccTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCgaga                                                                                          <  2:723184/139‑1 (MQ=255)                               ccagccTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCgaga                                                                                          <  1:503464/139‑1 (MQ=255)                                                gATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCagcagc                                                                         <  1:714520/139‑1 (MQ=255)                                                 aTCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCagcagc                                                                         <  2:737814/138‑1 (MQ=255)                                                 aTCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCagcagc                                                                         >  1:737814/1‑138 (MQ=255)                                                 aTCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGcc                                                                        >  2:502539/1‑139 (MQ=255)                                                 aTCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGcc                                                                        >  1:60996/1‑139 (MQ=255)                                                   cAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGcccc                                                                      <  2:220006/139‑1 (MQ=255)                                                         cGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCACCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCgataga                                                                >  1:215078/1‑139 (MQ=255)                                                                      gAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATc                                                   <  1:502539/139‑1 (MQ=255)                                                                            tgtATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGAc                                                                                                                   <  1:500167/69‑1 (MQ=255)                                                                            tgtATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGAc                                                                                                                   >  2:500167/1‑69 (MQ=255)                                                                            tgtATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATcc                                             >  2:598149/1‑139 (MQ=255)                                                                              tATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCgat                                                                   <  1:635587/115‑1 (MQ=255)                                                                              tATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCgat                                                                   >  2:635587/1‑115 (MQ=255)                                                                              tATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCa                                           >  1:264460/1‑139 (MQ=255)                                                                                      tgtCTACTTTCTTCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTg                                   <  1:151544/139‑1 (MQ=255)                                                                                      tgtCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTg                                   >  1:192741/1‑139 (MQ=255)                                                                                      tgtCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTg                                   <  2:801327/139‑1 (MQ=255)                                                                                        tCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGccc                                                                       <  2:52771/101‑1 (MQ=255)                                                                                        tCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGccc                                                                       >  1:52771/1‑101 (MQ=255)                                                                                        tCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCa                                 <  1:598149/139‑1 (MQ=255)                                                                                        tCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCa                                 >  1:118021/1‑139 (MQ=255)                                                                                        tCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCACCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCa                                 <  2:215078/139‑1 (MQ=255)                                                                                           aCTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCt                              <  2:192741/139‑1 (MQ=255)                                                                                               tCATCCGTCCAGGTTCCGGGGTTACTGGAGTCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTtaa                          >  2:535976/1‑139 (MQ=255)                                                                                                      tCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTGGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCa                                 >  1:530145/1‑125 (MQ=255)                                                                                                      tCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCa                                           <  2:42685/115‑1 (MQ=255)                                                                                                      tCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCa                                           >  1:42685/1‑115 (MQ=255)                                                                                                      tCCAGGTTCCGGGGTTACTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCa                                 <  2:530145/125‑1 (MQ=255)                                                                                                                       cTGGAATCCATCTAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTcc  >  2:295837/1‑139 (MQ=255)                                                                                                                            atccatctAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCa                  >  1:724671/1‑118 (MQ=255)                                                                                                                            atccatctAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCa                  <  2:724671/118‑1 (MQ=255)                                                                                                                                   |                                                                                                                               TCCCAGGCGGTAATTGGGTTCGTTGAGGAGCCAGCCTTTGATATTCAGATCAAATTCGTTGGCATAATTGAGTTGTGTATCAGGGTGTCTACTTTCATCCGTCCAGGTTCCGGGGTTACTGGAATCCATCCAGTCCTGATCGACCATATTGCCACCTCGGCTGCCGAGAGTTGTCCAGCCAGCAGCCCCGATAGATATCTGGGGCATCAAATCCCAATTAATTGCACCTTTAATAATTGCAGCGTTATTGAATTTCC  >  NC_000913/585187‑585443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑