Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 600,470 C→T A586V (GCG→GTG)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913600,4700CT100.0% 84.5 / NA 25A586V (GCG→GTG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

TGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCTTA  >  NC_000913/600353‑600604
                                                                                                                     |                                                                                                                                      
tGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGa                                                                                                                   <  2:378656/139‑1 (MQ=255)
  cTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa                                                                                                                 <  1:353144/139‑1 (MQ=255)
  cTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa                                                                                                                 <  1:770944/139‑1 (MQ=255)
  cTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa                                                                                                                 <  1:495216/139‑1 (MQ=255)
               ggCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTCTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg                                                                                                    <  2:425320/139‑1 (MQ=255)
                                 gCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCt                                                                                                             >  1:771961/1‑112 (MQ=255)
                                 gCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCt                                                                                                             <  2:771961/112‑1 (MQ=255)
                                                               gCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa                                                                                                                 <  2:386312/78‑1 (MQ=255)
                                                               gCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACa                                                                                                                 >  1:386312/1‑78 (MQ=255)
                                                               gCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAAc                                                    >  1:406830/1‑139 (MQ=255)
                                                                              tGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGc                                     >  1:153227/1‑139 (MQ=255)
                                                                               gCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCt                                    <  2:357008/139‑1 (MQ=255)
                                                                               gCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCt                                    >  1:357008/1‑139 (MQ=255)
                                                                                 cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAg                                                                                                               >  1:738791/1‑62 (MQ=255)
                                                                                 cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAg                                                                                                               <  2:738791/62‑1 (MQ=255)
                                                                                 cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg                                                                                                    >  1:684070/1‑73 (MQ=255)
                                                                                 cATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAg                                                                                                    <  2:684070/73‑1 (MQ=255)
                                                                                    cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGt                                                                                                 <  2:51730/73‑1 (MQ=255)
                                                                                    cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGt                                                                                                 >  1:51730/1‑73 (MQ=255)
                                                                                    cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGt                                                                                        <  1:625471/82‑1 (MQ=255)
                                                                                    cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGt                                                                                        >  2:625471/1‑82 (MQ=255)
                                                                                    cGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCt                               >  1:16418/1‑139 (MQ=255)
                                                                                         cTGCCGGGGATTTCCGCAGCAGAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGgaga                          <  2:16418/139‑1 (MQ=255)
                                                                                                          agcagAGGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATc         >  1:104271/1‑139 (MQ=255)
                                                                                                               agGCGGTGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCt    >  2:456081/1‑139 (MQ=255)
                                                                                                                 gcggtgAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCTTa  <  2:104271/139‑1 (MQ=255)
                                                                                                                     |                                                                                                                                      
TGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGCTGCCGGGGATTTCCGCAGCAGAGGCGGCGAGTATGCTGCAAAAAACCGACAAGCTAATTATGAGCGTACCTGAAGTGGCGCGGGTATTTGGCAAAACCGGGAAAGCGGAAACCGCCACCGATTCTGCTCCGCTGGAGATGGTAGAAACGACCATCCAGCTTA  >  NC_000913/600353‑600604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: