Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 738,087 A→G intergenic (+126/‑5) ybfL → / → ybfD pseudogene, DDE domain transposase family,putative factor, Not classified, putative receptor protein/H repeat‑associated putative transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913738,0870AG100.0% 178.8 / NA 56intergenic (+126/‑5)ybfL/ybfDpseudogene, DDE domain transposase family,putative factor, Not classified, putative receptor protein/H repeat‑associated putative transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/27);  total (29/27)

GGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGC  >  NC_000913/737951‑738220
                                                                                                                                        |                                                                                                                                     
ggCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCa                                                                                                                                     <  1:563161/139‑1 (MQ=255)
                 ccTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTaaaaaa                                                                                                                    >  1:663229/1‑139 (MQ=255)
                     cTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGa                                                                                                                >  1:404756/1‑139 (MQ=255)
                      tttttGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCCTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAGATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGAt                                                                                                               <  1:91925/139‑1 (MQ=255)
                       ttttGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATg                                                                                                              <  1:740542/139‑1 (MQ=38)
                            tACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACa                                                                                                         <  2:788787/139‑1 (MQ=25)
                            tACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACa                                                                                                         <  2:663229/139‑1 (MQ=37)
                            tACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACa                                                                                                         <  2:39771/139‑1 (MQ=37)
                               tAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACatat                                                                                                      >  2:97947/1‑139 (MQ=21)
                                aaaTAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGaa                                                                                                           <  2:675844/133‑1 (MQ=25)
                                aaaTAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGaa                                                                                                           >  1:675844/1‑133 (MQ=25)
                                aaaTAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATAtt                                                                                                     >  1:404090/1‑139 (MQ=21)
                                aaaTAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATAtt                                                                                                     >  1:595947/1‑139 (MQ=21)
                                           aTTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTACTTACATAATTAAGTAGAGCAAAATGAAACTTAAAAAATAGATGGAACATATTTCTATTATcac                                                                                          >  1:544558/1‑137 (MQ=11)
                                                     tAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGaa                                                                                                           >  1:89204/1‑112 (MQ=37)
                                                     tAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGaa                                                                                                           <  2:89204/112‑1 (MQ=255)
                                                     tAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                <  2:404090/139‑1 (MQ=21)
                                                     tAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                <  1:248817/139‑1 (MQ=25)
                                                                atatTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGaa                                                                     >  2:134680/1‑139 (MQ=37)
                                                                  atTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAg                                                                   <  2:144226/139‑1 (MQ=255)
                                                                        tattaCCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAAc                                                             >  1:469594/1‑139 (MQ=255)
                                                                             ccTGAAGCTGTTTTTTATTGCTTATACATGATAAAATACTTCTTACATAATTAAGGAGAGCAAAATGTAACTTAAAAAATTGATGGGACATATTACTATTATCCCCGATTACAGACAAGCCTGAAAATAAGAACAGaaa                                                        >  1:32478/1‑139 (MQ=11)
                                                                               tGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAAtt                                                      <  2:595947/139‑1 (MQ=255)
                                                                                 aaGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTAt                                                    >  2:307804/1‑139 (MQ=255)
                                                                                  aGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATc                                                   <  2:168819/139‑1 (MQ=38)
                                                                                     tGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGa                                                <  2:469594/139‑1 (MQ=38)
                                                                                     tGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGa                                                <  2:404756/139‑1 (MQ=255)
                                                                                       ttttttATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCgg                                                 <  1:458142/136‑1 (MQ=255)
                                                                                       ttttttATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCgg                                                 >  2:458142/1‑136 (MQ=37)
                                                                                             aTTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATccc                                                                                          >  2:719166/1‑89 (MQ=255)
                                                                                             aTTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATccc                                                                                          <  1:719166/89‑1 (MQ=255)
                                                                                                    ataCATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                >  1:57651/1‑92 (MQ=37)
                                                                                                    ataCATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                <  2:57651/92‑1 (MQ=37)
                                                                                                    ataCATGATCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGAc                                 >  1:342144/1‑139 (MQ=37)
                                                                                                           aTCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACa                                                                                                         <  2:150933/60‑1 (MQ=255)
                                                                                                           aTCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACa                                                                                                         >  1:150933/1‑60 (MQ=255)
                                                                                                           aTCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                >  2:312023/1‑85 (MQ=255)
                                                                                                           aTCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                <  1:312023/85‑1 (MQ=255)
                                                                                                            tCAAATACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgtg                         >  1:268019/1‑139 (MQ=255)
                                                                                                                aTACTTCTTACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGt                     >  2:229370/1‑139 (MQ=255)
                                                                                                                    ttcttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTAtt                 >  1:20027/1‑139 (MQ=37)
                                                                                                                     tcttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTAttt                >  2:73205/1‑139 (MQ=25)
                                                                                                                      cttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTa                                         <  2:454109/113‑1 (MQ=37)
                                                                                                                      cttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTa                                         >  1:454109/1‑113 (MQ=38)
                                                                                                                      cttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTc               >  2:372183/1‑139 (MQ=21)
                                                                                                                      cttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTc               >  2:397770/1‑139 (MQ=37)
                                                                                                                      cttACATAATTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACAATATGTGCCGTTAttac               >  1:587710/1‑137 (MQ=255)
                                                                                                                             aaTTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCa        <  2:587710/139‑1 (MQ=17)
                                                                                                                              aTTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCgg                                                 <  1:179874/97‑1 (MQ=21)
                                                                                                                              aTTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCgg                                                 >  2:179874/1‑97 (MQ=21)
                                                                                                                              aTTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTa                                         <  2:359657/105‑1 (MQ=17)
                                                                                                                              aTTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTa                                         >  1:359657/1‑105 (MQ=18)
                                                                                                                              aTTAAGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAg       >  2:127618/1‑139 (MQ=17)
                                                                                                                                  aGGAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAgg   <  1:307804/139‑1 (MQ=17)
                                                                                                                                   ggAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGc  <  2:268019/139‑1 (MQ=18)
                                                                                                                                   ggAGAGCAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGc  <  1:229370/139‑1 (MQ=18)
                                                                                                                                        |                                                                                                                                     
GGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTTCTTACATAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAGCCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGC  >  NC_000913/737951‑738220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: