Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 857,298 C→T R446W (CGG→TGG)  ybiT → ABC‑F family putative regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,2980CT100.0% 155.2 / NA 46R446W (CGG→TGG) ybiTABC‑F family putative regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/29);  total (17/29)

GTGTTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCT  >  NC_000913/857163‑857430
                                                                                                                                       |                                                                                                                                    
gtgtTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGa                                                                                                                                   <  2:343382/139‑1 (MQ=255)
   ttCGAATGGATGAGCCAGTGGAAGCAGGAAGGTGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGAt                                                                                                                                  <  2:94926/137‑1 (MQ=255)
   ttCGAATGGATGAGCCAGTGGAAGCAGGAAGGTGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGAt                                                                                                                                  >  1:94926/1‑137 (MQ=255)
          ggATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                                         <  1:126093/139‑1 (MQ=255)
          ggATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                                         <  1:225957/139‑1 (MQ=255)
           gATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGt                                                                                                                        >  2:183693/1‑139 (MQ=255)
             tGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTaa                                                                                                                      >  2:410039/1‑139 (MQ=255)
               aGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGt                                                                                                                    <  1:339738/139‑1 (MQ=255)
                          gCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGGTTGGTAAGTTAATGATGCAg                                                                                                         >  1:219350/1‑139 (MQ=255)
                              gAACGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGc                                                                                                     >  2:711608/1‑139 (MQ=255)
                                 ggCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGa                                                                                                  <  2:654220/139‑1 (MQ=255)
                                      tGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACACCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACAtt                                                                                             <  1:192814/139‑1 (MQ=255)
                                        aCGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCt                                                                                           <  1:183693/139‑1 (MQ=255)
                                                      cGCAGTATTCTCGGTCGTTTTCTGTTCAGGCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAAc                                                                             <  1:711608/139‑1 (MQ=255)
                                                      cGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAAc                                                                             >  2:615228/1‑139 (MQ=255)
                                                                tcGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGcc                                                                                                    <  2:574052/106‑1 (MQ=255)
                                                                tcGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGaccaacca                                                                   >  2:547973/1‑139 (MQ=255)
                                                                tcGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATACTGTTTGGTAAGTTAATGATGCAGAAGcc                                                                                                    >  1:574052/1‑106 (MQ=255)
                                                                 cGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAaccac                                                                  <  2:219350/139‑1 (MQ=255)
                                                                   gTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCt                                                                <  2:626288/139‑1 (MQ=255)
                                                                    tCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGATGAACCGACCAACCACCTg                                                               <  2:28742/139‑1 (MQ=255)
                                                                     cGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTgg                                                              >  1:338887/1‑139 (MQ=255)
                                                                     cGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTgg                                                              >  1:32378/1‑139 (MQ=255)
                                                                       tttGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGat                                                            >  1:324420/1‑139 (MQ=255)
                                                                       tttGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGat                                                            <  1:461240/139‑1 (MQ=255)
                                                                                   ccAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCAtt                                                >  2:688396/1‑139 (MQ=255)
                                                                                      ggACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAg                                             <  1:723296/139‑1 (MQ=255)
                                                                                        acgacATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATgg                                                                                   <  2:515615/99‑1 (MQ=255)
                                                                                        acgacATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGAGCATgg                                                                                   >  1:515615/1‑99 (MQ=255)
                                                                                             aTCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                                         <  1:733531/56‑1 (MQ=255)
                                                                                             aTCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTgg                                                                                                                         >  2:733531/1‑56 (MQ=255)
                                                                                                 aaaaGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACAt                                  <  2:338887/139‑1 (MQ=255)
                                                                                                       cAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACt                            <  1:547973/139‑1 (MQ=255)
                                                                                                        aGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACAtt                                                                                             <  1:671782/73‑1 (MQ=255)
                                                                                                        aGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACAtt                                                                                             >  2:671782/1‑73 (MQ=255)
                                                                                                         gCTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTgg                          >  1:80574/1‑139 (MQ=255)
                                                                                                          cTAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGa                         <  1:615228/139‑1 (MQ=255)
                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa                        <  1:410039/139‑1 (MQ=255)
                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa                        <  2:479583/139‑1 (MQ=255)
                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa                        <  2:324420/139‑1 (MQ=255)
                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa                        <  2:769759/139‑1 (MQ=255)
                                                                                                           tAAAGTGCTTTCCGGTGGGGAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGaa                        <  2:32378/139‑1 (MQ=255)
                                                                                                                     tCCGGTGGGGACAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCCGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAgg              <  1:372815/139‑1 (MQ=255)
                                                                                                                              gAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTgg                          >  2:547997/1‑118 (MQ=255)
                                                                                                                              gAAAAAGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTgg                          <  1:547997/118‑1 (MQ=255)
                                                                                                                                 aaaGGGTGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCt  <  2:80574/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTGTTCGAATGGATGAGCCAGTGGAAGCAGGAAGGCGATGACGAGCAGGCGGTACGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCT  >  NC_000913/857163‑857430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑