Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 936,202 C→T T993T (ACC→ACT ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913936,2020CT88.0% 59.7 / 2.7 25T993T (ACC→ACTftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (11/11);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

CGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGC  >  NC_000913/936085‑936332
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cGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGgt                                                                                                               >  2:264670/1‑139 (MQ=255)
          cgcgATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCga                                                                                                     <  2:593609/139‑1 (MQ=255)
          cgcgATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCga                                                                                                     >  2:247208/1‑139 (MQ=255)
                     gTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGTCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGc                                                                                          <  1:139175/139‑1 (MQ=255)
                                   tggtgCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTATATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGc                                                                            <  1:247208/139‑1 (MQ=255)
                                      tgCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGcaca                                                                         <  2:407592/139‑1 (MQ=255)
                                        cTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGc                                                                       >  2:652516/1‑139 (MQ=255)
                                                aGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGAt                                                               >  2:506708/1‑139 (MQ=255)
                                                aGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGAt                                                               >  2:43487/1‑139 (MQ=255)
                                                aGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGAt                                                               >  1:513200/1‑139 (MQ=255)
                                                      cGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGt                                                         <  2:720707/139‑1 (MQ=255)
                                                      cGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGt                                                         >  2:751493/1‑139 (MQ=255)
                                                       gCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGtt                                                        <  2:299906/139‑1 (MQ=255)
                                                       gCCGGTGAGACGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGtt                                                        <  2:513200/139‑1 (MQ=255)
                                                            tGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGaa                                                                                                          <  1:486949/84‑1 (MQ=255)
                                                            tGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCAGTCGGTGTGaa                                                                                                          >  2:486949/1‑84 (MQ=255)
                                                                                       aaTGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGt                                                         <  2:422050/106‑1 (MQ=255)
                                                                                       aaTGCCGCACTTGTTGGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGt                                                         >  1:422050/1‑106 (MQ=255)
                                                                                                 ttgttgGTTGCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATg              <  1:506708/139‑1 (MQ=255)
                                                                                                         tgCGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCAtt      >  1:285140/1‑139 (MQ=255)
                                                                                                         tgCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGAt                                              >  2:131476/1‑99 (MQ=255)
                                                                                                         tgCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGAt                                              <  1:131476/99‑1 (MQ=255)
                                                                                                           cGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCg                                                          >  2:129462/1‑85 (MQ=255)
                                                                                                           cGGGGACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCg                                                          <  1:129462/85‑1 (MQ=255)
                                                                                                             gggACTACTGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGc  >  2:684937/1‑139 (MQ=255)
                                                                                                                     |                                                                                                                                  
CGCCAAATTCCGCGATAATCCGTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGC  >  NC_000913/936085‑936332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: