Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,036,083 C→T A105V (GCG→GTG)  hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,036,0830CT100.0% 79.5 / NA 24A105V (GCG→GTG) hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/9);  total (15/9)

TCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGC  >  NC_000913/1035958‑1036217
                                                                                                                             |                                                                                                                                      
tctcGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATc                                                                                                                           >  2:36612/1‑139 (MQ=255)
     aTGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCgg                                                                                                                      >  2:197805/1‑139 (MQ=255)
      tGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGa                                                                                                                     <  2:405451/139‑1 (MQ=255)
          aTTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATc                                                                                                                 <  2:77158/139‑1 (MQ=255)
                  cGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGgtgc                                                                                                                                      <  1:404240/110‑1 (MQ=255)
                  cGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGgtgc                                                                                                                                      >  2:404240/1‑110 (MQ=255)
                                    gCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGt                                                                                       <  2:283674/139‑1 (MQ=255)
                                                          cGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgacg                                                                 >  2:648108/1‑139 (MQ=255)
                                                                              cgcgAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCaa                                                                          <  2:768646/110‑1 (MQ=255)
                                                                              cgcgAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCaa                                                                          >  1:768646/1‑110 (MQ=255)
                                                                                  aagaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAAc                                         >  1:163592/1‑139 (MQ=255)
                                                                                   agaaAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACt                                        >  2:462768/1‑139 (MQ=255)
                                                                                             ccTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                           <  2:242841/62‑1 (MQ=255)
                                                                                             ccTGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAg                                                                                                           >  1:242841/1‑62 (MQ=255)
                                                                                               tGCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCa                            >  2:190401/1‑139 (MQ=255)
                                                                                                gCATCAGAACAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCaa                           >  1:608157/1‑139 (MQ=255)
                                                                                                       aaCAGTTTCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCg                    >  1:62626/1‑139 (MQ=255)
                                                                                                            tttCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGcc                                                                                                             >  2:293676/1‑45 (MQ=255)
                                                                                                            tttCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGcc                                                                                                             <  1:293676/45‑1 (MQ=255)
                                                                                                            tttCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCAc                                   >  1:698358/1‑119 (MQ=255)
                                                                                                            tttCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCAc                                   <  2:698358/119‑1 (MQ=255)
                                                                                                            tttCTCCGAAGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCg               >  1:382117/1‑139 (MQ=255)
                                                                                                                    aaGTGTTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAggc       <  2:309634/139‑1 (MQ=255)
                                                                                                                      gtgtTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAggc       <  1:455186/137‑1 (MQ=255)
                                                                                                                      gtgtTGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAggc       >  2:455186/1‑137 (MQ=255)
                                                                                                                         ttGGTGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGgcgc  >  1:240144/1‑139 (MQ=255)
                                                                                                                             |                                                                                                                                      
TCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGC  >  NC_000913/1035958‑1036217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: