Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,276,337 G→A R428C (CGC→TGC)  narX ← sensory histidine kinase in two‑component regulatory system with NarL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,276,3370GA100.0% 148.7 / NA 44R428C (CGC→TGC) narXsensory histidine kinase in two‑component regulatory system with NarL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/27);  total (17/27)

GCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAA  >  NC_000913/1276212‑1276466
                                                                                                                             |                                                                                                                                 
gCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGc                                                                                                                      >  1:84921/1‑139 (MQ=255)
  aaCTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAg                                                                                                                    >  1:329411/1‑139 (MQ=255)
    cTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAgcg                                                                                                                  >  2:551831/1‑139 (MQ=255)
      cGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCa                                                                                                                <  2:568884/139‑1 (MQ=255)
       gCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCAt                                                                                                               <  2:703499/139‑1 (MQ=255)
          tCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGc                                                                                                            <  1:350129/139‑1 (MQ=255)
            cAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGccc                                                                                                          <  2:171909/139‑1 (MQ=255)
              gcgcCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTg                                                                                                        <  2:63901/139‑1 (MQ=255)
                 ccGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCAt                                                                                                     <  2:479345/139‑1 (MQ=255)
                                     gTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCAt                                                                                                               >  1:387320/1‑109 (MQ=255)
                                     gTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCAt                                                                                                               <  2:387320/109‑1 (MQ=255)
                                     gTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCGGCa                                                                                 >  2:516263/1‑139 (MQ=255)
                                      tGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCTTTCAGTTCGTTACGGATCCGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCAt                                                                                <  1:141931/139‑1 (MQ=255)
                                      tGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCAt                                                                                <  1:434160/139‑1 (MQ=255)
                                             cAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATg                                                                         <  2:329411/139‑1 (MQ=255)
                                              aaGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGc                                                                        <  1:714398/139‑1 (MQ=255)
                                                   gAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCACTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagag                                                                   <  1:572033/139‑1 (MQ=255)
                                                     aTGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagagag                                                                 <  2:701573/139‑1 (MQ=255)
                                                                  ttCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATa                                                    <  2:660551/139‑1 (MQ=255)
                                                                              cgcCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGt                                        >  2:779241/1‑139 (MQ=255)
                                                                                cccAGGATGCATTCAGTTGGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTc                                      >  1:443978/1‑139 (MQ=255)
                                                                                           ttCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCAt                                                                                                     <  2:214458/65‑1 (MQ=255)
                                                                                           ttCAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCAt                                                                                                     >  1:214458/1‑65 (MQ=255)
                                                                                             cAGTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAAtggtg                         <  1:368015/139‑1 (MQ=255)
                                                                                               gTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCaa                              >  2:562366/1‑132 (MQ=255)
                                                                                               gTTCGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCaa                              <  1:562366/132‑1 (MQ=255)
                                                                                                  cGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTACACAACTCACCTGCATCGTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGTACg                    <  1:516263/139‑1 (MQ=255)
                                                                                                  cGTTACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACg                    <  1:779241/139‑1 (MQ=255)
                                                                                                    ttACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGct                  >  1:543365/1‑139 (MQ=255)
                                                                                                     tACGGATCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGctc                 >  2:404285/1‑139 (MQ=255)
                                                                                                          aTCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTcgcg                                   <  2:307918/116‑1 (MQ=255)
                                                                                                          aTCTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTcgcg                                   >  1:307918/1‑116 (MQ=255)
                                                                                                            cTGACTTAACAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATc          <  2:543365/139‑1 (MQ=255)
                                                                                                                    aCAGTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCaa  <  1:398267/139‑1 (MQ=255)
                                                                                                                       gTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTc                                      <  2:707651/100‑1 (MQ=255)
                                                                                                                       gTTCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTc                                      >  1:707651/1‑100 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACt                                                                                         >  1:555603/1‑47 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACt                                                                                         <  2:555603/47‑1 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagag                                                                   >  1:206216/1‑69 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagag                                                                   <  1:503756/69‑1 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagag                                                                   >  2:503756/1‑69 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAagag                                                                   <  2:206216/69‑1 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTg                                                           >  2:774509/1‑77 (MQ=255)
                                                                                                                         tCGCAGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTg                                                           <  1:774509/77‑1 (MQ=255)
                                                                                                                             |                                                                                                                                 
GCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCATGCAGTTCGCGCGCAATGGTGGCACGCTCTTCCATCACGATCAA  >  NC_000913/1276212‑1276466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: